Acronym AquaCrispr
Category
Marine Biotechnology
Title Optimalization of the CRISPR/CAS9 knock-in technology and application in salmon and trout Programme International Cooperation
Instrument (FP6)
Contact Type (FP7)
Strand (Interreg)
NA
Theme (FP7)
Activity Area (FP6)
Regional Area (Interreg)
Action (COST)
NA
Specific Programme (FP7)
NA
Funding source National-European Coordinator Rolf Edvardsen Coordinator email NA
Coordinator institution
IMR - Institute of Marine Research (Norway)
Institutions involved
INRA - French National Institute for Agricultural Research (France) ,
MPI - Max Planck Institute for Biology Tübingen (Germany) ,
Start year 2017 End year 2020 Funding (€) € 1,755,000 Website NA Summary "The primary aim of the project is to optimize the CRISPR/Cas9 knock-in technology in zebrafish and establish the protocol for salmon and trout.
The secondary aim is to utilize these tools to explain mechanisms behind some key biological features in fish such as time of maturity (Ayllon et al. 2015), pigmentation (Irion et al. 2016), immune functions (Kjærner-Semb et al. 2016), sex determination (Herpin and Schartl 2011, Herpin and Schartl 2015), reproduction (Taranger et al.2010)and egg quality(Bobe and Labbe 2010).
In order to achievethis we will build on exsiting expertice in the consortiumon knock-in in zebrafish and established protocols for microinjection and CRISPR/Cas9 knock-out in the salmonides developed by the consortium partners.
The main objectives to accomplish the aim will be:
1. Improvement of the knock-in protocol in zebrafish (primarily) and Medaka by testing different designs of donor DNA constructs and Cas9 proteins;
2. Implementing the best practices for knock-in from zebrafish and Medaka to salmon and trout;
3. Utlizing knock-in of putative important genetic variants in salmon and trout. Our hypothesis is that the enhanced CRISP Rmethodology will be transferable from the model species to farmeds pecies,enabling functional studies of key life history traits,which will ultimately lead to genetically improved aquaculture fish. Results from this will generate a larger knowledge base for aquaculture and also explore whether some of these traits may be transferable to farming of salmonids."
Keywords
Engineering;
Salmon;
Fish;
Trout;
Genetic;
Marine Region
76
Not associated to marine areas
0
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