Acronym NA
Category
Marine Biotechnology
Title Single cell oil PUFA production by food rest materials Programme National Programme
Instrument (FP6)
Contact Type (FP7)
Strand (Interreg)
NA
Theme (FP7)
Activity Area (FP6)
Regional Area (Interreg)
Action (COST)
NA
Specific Programme (FP7)
NA
Funding source National Coordinator Achim Kohler Coordinator email achim.kohler@nmbu.no
Coordinator institution
NA
Institutions involved
NA
Start year 2014 End year 2016 Funding (€) € 502,400 Website https://prosjektbanken.forskningsradet.no/en/project/FORISS/234258?Kilde=FORISS&distribution=Ar&chart=bar&calcType=funding&Sprak=no&sortBy=date&sortOrder=desc&resultCount=30&offset=150&TemaEmne.2=Industriell+bioteknologi Summary There is a growing interest in sources of long-chain polyunsaturated fatty acids (PUFA) that represent alternatives to fish oil. The main focus is on microorganisms as sources of edible lipids, the single cell oils (SCO), due to their ability to produce lipids that are rarely found in the plant or animal sources. Filamentous fungi show a greater diversity of lipid types and fatty acids than other microorganisms. In addition, due to their ability to utilize a broad range of substrates, wastes of food proc essing industry can be used as a cheap substrate/cultivation medium for fungal fermentation.
The aim of the SingleCellOil project is to apply a high-throughput micro-bioreactor system and FTIR spectroscopy for selection of fungal strains with naturally high PUFA content and to optimize substrates based on food processing wastes (chicken by-products, potato peel, eggshells and fish by-products) for fungal fermentation. The main focus will be put on optimization of fermentation parameters, extraction and pu rification procedures towards a high yield of PUFA.
There are four main research and development activities that the industry partners want to outsource to the RTD partners. The first is the high-throughput screening of fungi in order to establish a libr ary of PUFA producing fungi. The second is the optimization of food processing wastes to be sued as a cultivation medium in fungal fermentation. The third is an extensive screening of the library of oleaginous fungi with substrates derived from different food processing wastes. Finally, the fourth activity is the optimization of fermentation conditions on lab/industrial scale and the development of extraction and purification procedures. These four innovations combined will allow performing value added ut ilization of food processing wastes and creating a new marketable product: single cell oil PUFA.
Goal:
In order to utilize fungal production of rest materials from food industry in Norway, there is a need of research to
1. Establish processes that transform the existing rest materials into suitable fermentation media
2. Screen fungal strains in order to identify strains that optimize PUFA production in industrial processes
3. Optimize lipid extraction and purification process on both lab and industrial scale.
This requires extensive screening studies involving hundreds of fungal strains and high numbers of test fermentations, and, not least, analytical tools for monitoring the production of PUFAs. Recently, we together with several partners developed a fully automated system by which several hundred fermentations (up to 1584 fermentations) can be performed in parallel and by which PUFA production can be monitored by FTIR spectroscopy in each single fermentation. Thus, by this system large numbers of conditions can be screened allowing an effective optimization of single cell oil processes. This fully automated system for monitoring PUFA in high-throughput cultivation is unique world-wide and provides a clear advantage for the Norwegian industry in the development of such biotechnological industrial processes.
Keywords
Waste valorization;
Wastes;
Fungus;
Monitoring;
Bioprospecting;
Byproducts;
Bioproduct;
Fish oil replacement;
Technology;
Marine Region
76
Not associated to marine areas
0
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