Acronym IDASSMyx
Category
Aquaculture
Title Infection dynamic of Aquaculture Seabass and Seabream by Myxozoa Programme Nat. Programme (supported by ESIF)
Instrument (FP6)
Contact Type (FP7)
Strand (Interreg)
NA
Theme (FP7)
Activity Area (FP6)
Regional Area (Interreg)
Action (COST)
NA
Specific Programme (FP7)
NA
Funding source National-European Coordinator Maria Joao Faria Leite Dias dos Santos Coordinator email mjsantos@fc.up.pt
Coordinator institution
CIIMAR - Interdisciplinary Centre of Marine and Environmental Research (Portugal)
Institutions involved
ISCS-N - Advanced Institute of Health Sciences - North (Portugal) ,
CESPU - Cooperative Higher Education, Polytechnic and University (Portugal) ,
MTA - Hungarian Academy of Sciences (Hungary) ,
MTA-VMRI - Hungarian Academy of Sciences; Fish Pathology Research Team (Hungary) ,
MTA-VMRI - Hungarian Academy of Sciences; Institute for Veterinary Medical Research (Hungary) ,
ICBAS - Institute of Biomedical Sciences Abel Salazar (Portugal) ,
UP - University of Porto (Portugal) ,
Start year 2012 End year 2015 Funding (€) € 123,767 Website http://mjsantos.wix.com/idassmyx Summary The Myxozoa infecting the European seabass and seabream cause serious growing delays, with great economic losses. In Portugal at least 4 Myxozoa species have been reported (Sphaerospora dicentrachi, Ceratomyxa labracis, C. dicentrachi and Myxobilatus sp.) which occur quite frequently and relatively little is known about their life cycle. No systematic patasitological surveys have been conducted on seabream in Portugal and none of the recorded Myxozoa species life cycle has been established abroad. The project aims to determine the life cycle of Myxozoa, so that in the future infections can be controlled and measures can be proposed based on this new knowledge. The Myxozoa taxon includes more than 1400 species, already described morphologically. Life cycles for Myxozoan species in a few freshwater fish species have been established. Thus the present work will be internationally pioneering in the field. The project will characterize the life cycle of the species of Myxozoa known in seabass and seabream: providing important information on their ultrastructure; performing the molecular characterization of the forms actinosporean and myxosporean in order to match them and, that way, close their life cycles, and to provide an efficient tool of diagnosis for each parasitosis; and also to describe the development stages of Actinoporea in Annelida and test some infection conditions. Samples of seabass and seabream, and other occasional fish, in aquaculture that produces fish in a semi-intensive way, in Algarve, in order to obtain infected tissues by species of Myxozoa (Myxosporean form) and thus to study its ultrastructure and perform its molecular characterization. In the same locality and in the same fish pounds, we will perform periodical samples of Annelida, during 2 years, where it will be surveyed the actinosporean forms, characterize its morphology (namely its ultrastructure) and genotype, and studied its development in this host. At the same time we will do experimental infections of Annelida, with myxosporean spores, where the infection relationship with the host sex and size will be evaluated. Lastly, using the molecular information, which will be sent to the GenBank, we will try to match the species found, either in the form of Actinosporea and Myxosporea, with other genotypes available in order to find pairs and thus close the life cycle of some species. The fact that we are studying forms of actinosporean and myxosporean present in the same place increase the probability of that matching, since it is highly probable that all life cycle occurs in the same environment. From our experience with works in the same field, we can inform that in 2 species of Polychaetes studied we report the occurrence of 2 different species of actinosporean. Both species have already being presented to the scientific community (Rangel et al 2009, in press) and are now being studied in detail for their ultrastructure (TEM). For this later work, the methodology was already adjusted, since the protocol used for myxosporea did not show good results. With this project we intend to give institutional support to a collaboration among partners that has started some years ago.
To study the Myxozoa of seabass, seabream and annelids in a semi-intensive aquaculture system: (1) In first place we will sample seabass and seabream, and other occasional fish, in aquaculture that produces fish in a semi-intensive way, in Algarve, in order to obtain infected tissues by species of Myxozoa (Myxosporean form) and thus to study its ultrastructure and perform its molecular characterization; (2) In the same locality and in the same fish pounds, we will perform periodical samples of Annelida, during 2 years, where it will be surveyed the actinosporean forms, characterize its morphology (namely its ultrastructure) and genotype, and studied its development in this host. At the same time we will do experimental infections of Annelida, with myxosporean spores, where the infection relationship with the host sex and size will be evaluated; (3) At last, and using the molecular information, which will be send to the GenBank, we will try to match the species found, either in the form of Actinosporea and Myxosporea, with other genotypes available in order to find pairs and thus close the life cycle of some species. The fact that we are studying forms of actinosporean and myxosporean present in the same place increase the probability of that matching, since it is highly probable that all life cycle occurs in the same environment.
Keywords
Genetic;
Fish;
Seabream;
Fish health;
Life cycle;
Parasite;
Seabass;
Disease;
Marine Region
38
Portuguese Waters (27.IXa,27.IXb)
1
Marine Region Map
