Acronym NA
Category
Aquaculture
Title Genomikk mot IPN - markørassistert seleksjon for umiddelbar forbedring av IPN-status i regnbueørretoppdrett - Genomics against IPN - marker-assisted selection for immediate improvement of IPN status in rainbow trout farming Programme National Programme
Instrument (FP6)
Contact Type (FP7)
Strand (Interreg)
NA
Theme (FP7)
Activity Area (FP6)
Regional Area (Interreg)
Action (COST)
NA
Specific Programme (FP7)
NA
Funding source National Coordinator Thomas Moen Coordinator email historisk-thomas.moen@nofima.no
Coordinator institution
NOFIMA - Norwegian Institute of Food, Fisheries and Aquaculture Research (Norway)
Institutions involved
NA
Start year 2011 End year 2014 Funding (€) € 198,602 Website https://prosjektbanken.forskningsradet.no/en/project/FORISS/208338?Kilde=FORISS&distribution=Ar&chart=bar&calcType=funding&Sprak=no&sortBy=date&sortOrder=desc&resultCount=30&offset=150&ProgAkt.3=HAVBRUKS-Havbruk%20-%20en%20n%C3%A6ring%20i%20vekst Summary The viral disease infectious pancreatic necrosis (IPN) is one of the biggest loss factors in both salmon and rainbow trout farming. In 2009, the breeding company Aqua Gen AS launched salmon roe specially selected, using DNA markers, for extra high resistance to IPN. An ongoing evaluation project shows that the specially selected fish is virtually resistant to IPN, and the product has consequently attracted great attention in the salmon industry both nationally and internationally. In this project we want to continue this initiative to also include resistance to IPN in rainbow trout. To achieve this, we must 1) develop research tools that are currently lacking in this species and 2) identify the genes that affect the degree of IPN resistance in the species. One hundred rainbow trout from Aqua Gen will be sequenced with second-generation sequencing technology from Illumina, to a total sequence amount corresponding to 100x coverage of the genome. The dataset will be used to identify a large number of 'single nucleotide polymorphism' markers (SNPs) throughout the genome. Comparisons between this dataset, the Atlantic salmon whole genome sequence, and the brown trout sequence will be used to identify conserved, non-coding regions in the genome. SNPs in such areas, as well as SNPs in coding areas, will be prioritized in the production of a 'SNP chip' with 50,000 SNPs. This SNP chip will be genotyped on rainbow trout from an IPN infection test, and used to identify the genes that determine the degree of IPN resistance in rainbow trout. The identified genes will be implemented in Aqua Gen's production of roe from rainbow trout from 2012, in such a way that improved genetics can be brought directly into the industry. The end product will be roe from the rainbow trout, specially selected for extra high resistance to IPN
Keywords
Trout;
Disease;
Selective breeding;
Genetic;
Fish;
Genomic;
Marine Region
76
Not associated to marine areas
0
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