Acronym NA
Category
Marine Biotechnology
Title Host immunity and skin microbiome interplay: Importance for protection against sea lice infestation in Atlantic salmon Programme National Programme
Instrument (FP6)
Contact Type (FP7)
Strand (Interreg)
NA
Theme (FP7)
Activity Area (FP6)
Regional Area (Interreg)
Action (COST)
NA
Specific Programme (FP7)
NA
Funding source National Coordinator Øystein Evensen Coordinator email oystein.evensen@nmbu.no
Coordinator institution
NMBU - Norwegian University of Life Sciences (Norway)
Institutions involved
NA - Cermaq Norway AS (Norway) ,
NA - Grieg Seafood Finnmark AS (Norway) ,
IMR - Institute of Marine Research (Norway) ,
NA - Lerøy Norway Seafoods AS (Norway) ,
NVI - Norwegian Veterinary Institute (Norway) ,
UCSD - University of California (United States of America) ,
Start year 2019 End year 2022 Funding (€) € 571,950 Website https://www.fhf.no/prosjekter/prosjektbasen/901566/ Summary Previous attempts to develop vaccines against salmon lice infection in salmon have not produced satisfactory results. Candidate antigens are difficult to identify and test for their ability to induce efficacious immune responses. Several studies have used a concealed antigen strategy (transferred from the Boophilus microplus vaccine in cattle) and while results initially were promising, results have been difficult to reproduce. NMBU/Aqua has participated in the Sea Lice Research Center (SLRC) with a focus on immunomodulation of the host, including to develop immunoprophylactic principles against infection. We have worked with vaccination modalities using DNAprime/ protein boost but again, results have not been promising. For this reason we have taken a different path with an idea that lack of protection can be related to amount of antigen used for immunization/ low corresponding antibody responses. Lice antigens have been prepared from sea lice and used for immunization of salmon at high antigen load. With this approach we have obtained a 65% reduction in sea lice numbers (compared to controls) and a heavy infection load.
Main objective:
To establish a protocol for vaccination combined with skin microbiome engineering that increase the resistance towards lice infection in Atlantic salmon.
Sub-objectives:
• To identify protective antigens of lice preparations for subsequent use of recombinant antigen design and vaccine delivery using a ""reverse vaccinology"" approach.
• To characterize, quantify and detail the impact of sea lice infection on the skin microbiome in Atlantic salmon in terms of its taxon presence/absence and relative abundance.
• To test these prophylactic interventions at laboratory scale and in small scale field studies.
Keywords
Parasite;
Genetic;
Fish;
Microbiome;
Vaccines development;
Salmon;
Marine Region
76
Not associated to marine areas
0
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