Acronym NA
Category
Marine Biotechnology
Title Physical map for oysters using fluorescence in situ hybridization technique Programme National Programme
Instrument (FP6)
Contact Type (FP7)
Strand (Interreg)
NA
Theme (FP7)
Activity Area (FP6)
Regional Area (Interreg)
Action (COST)
NA
Specific Programme (FP7)
NA
Funding source National Coordinator Karine Bouilly Coordinator email kbouilly@yahoo.fr
Coordinator institution
UTAD - University of Tras-os-Montes and Alto Douro (Portugal)
Institutions involved
FCT - Foundation for Science and Technology (Portugal) ,
Start year 2008 End year 2011 Funding (€) € 127,910 Website https://www.fct.pt/apoios/projectos/consulta/vglobal_projecto?idProjecto=66143&idElemConcurso=860 Summary Genetic investigations in bivalves are of special interest because of the economic and ecological importance of this group of marine invertebrates. Several banding techniques were already applied to bivalve chromosomes and the recent development of fluorescence in situ hybridization (FISH) technique allows a great progress in bivalve molecular cytogenetics. The Pacific oyster, Crassostrea gigas has received increasing interest because of its aquacultural importance worldwide. This oyster species has 2n=20 chromosomes. Aneuploidy phenomenon has been observed in several bivalve populations, especially in C. gigas. Additionally, polyploid (3n, 4n) oysters have been obtained by using various methods and are commercially produced. As a consequence, genetic and genomic tools are of increasing importance to assist genetic improvement and sustainability of the production. Chromosome identification is essential in oyster genomic research. Fluorescence in situ hybridization (FISH) offers new opportunities for the identification of oyster chromosomes. Several kinds of FISH probes could be used to reach this objective. Simple sequence repeats (SSRs), satellite DNA sequences, microdissected chromosomes and clones from the Pacific oyster BAC library could be valuable markers for define chromosome regions. All these tools will allow us to individually identify chromosomes and chromosomal arms, and thus, to build a physical map in C. gigas as complete as possible. This project represents the first step towards the construction of a physical chromosome map for the Pacific oyster. It will be essential for aneuploidy studies and in analysis of oyster genome organization and evolution. In this project, we expect to apply the most recent molecular cytogenetics techniques: (1) To identify all the chromosome pairs in C. gigas; (2) To build a physical map for C. gigas.
(1) The physical organization of different simple sequence repeats (SSRs) in metaphase chromosomes of Crassostrea gigas; (2) The physical organization of satellite DNA sequences in Crassostrea gigas; (3) Construction of a chromosome-specific DNA library for oysters by laser microdissection; (4) Identification and characterization of BAC clones for use as FISH probes in Crassostrea gigas; (5) Construction of a physical map for Crassostrea gigas.
Keywords
Shellfish;
Genomic sequencing;
Bivalve;
Genetic;
Mollusc;
Technology;
Oyster;
Genomic;
Marine Region
76
Not associated to marine areas
0
Marine Region Map
