Acronym NA
Category
Marine Biotechnology
Title Resistance towards pyrethroids and emamectin in sea lice Programme National Programme
Instrument (FP6)
Contact Type (FP7)
Strand (Interreg)
NA
Theme (FP7)
Activity Area (FP6)
Regional Area (Interreg)
Action (COST)
NA
Specific Programme (FP7)
NA
Funding source National Coordinator Tor Einar Horsberg Coordinator email tor.e.horsberg@nvh.no
Coordinator institution
NVH - Norwegian School of Veterinary Science (Norway)
Institutions involved
NA
Start year 2008 End year 2011 Funding (€) € 506,463 Website https://prosjektbanken.forskningsradet.no/project/FORISS/184650?Kilde=FORISS&distribution=Ar&chart=bar&calcType=funding&Sprak=no&sortBy=date&sortOrder=desc&resultCount=30&offset=0&Organisasjon.3=NORGES%20VETERIN%C3%86RH%C3%98GSKOLE Summary Ectoparasitic copepods represents a critical economical and health related threat to the salmonid aquaculture industry, as well as to wild salmonids. The primary method of control is delousing infected fish with pyrethroids and avermectins. Resistance towards chemotherapeutants has been demonstrated in sealice. This project aims to elucidate the molecular and biochemical basis for resistance, investigate the extent and the time scale in which decreased sensitivity to these agents may develop and to develop rapid diagnostic tests for the detection of important resistance mechanisms. The group at the Norwegian School of Veterinary Science has detected a novel mutation (Q945R) in the para-sodium channel gene, possibly related to pyrethroid resistance. One main task in the project will be to elucidate the significance of this mutation and develop a diagnostic test. Furthermore, the project will examine a larger part of the para-sodium channel gene for other possible resistance-associated mutations. Another aim will be to isolate and clone the glutamate-gated chloride channel, the target protein of avermectins. Selection experiments will be implemented to investigate the time required for sealice to develop resistance under controlled selection conditions, potential cross resistance, and potential consequences on other fitness traits. Previously established bioassays will be utilised to quantify degrees of sensitivity. If resistance-associated mutations are detected, a rapid, diagnostic test will be developed. Yet another aim will be to study the role of ATP-dependent efflux pumps (ABC-transporters) in the development of reduced sensitivity. First, the genes for ABC transporters in these species will be identified and their sequences will be determined. Then, the qualitative and quantitative expression of ABC-transporters in various tissues will be elucidated, and the resulting transcription profiles after exposure to selected compounds will be determined by real time PCR
Keywords
Parasite;
Genomic sequencing;
Salmon;
Fish;
Engineering;
Genetic;
Marine Region
76
Not associated to marine areas
0
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