Acronym NA
Category
Aquaculture
Title Salmon louse vaccine - identification and evaluation of novel antigens Programme National Programme
Instrument (FP6)
Contact Type (FP7)
Strand (Interreg)
NA
Theme (FP7)
Activity Area (FP6)
Regional Area (Interreg)
Action (COST)
NA
Specific Programme (FP7)
NA
Funding source National Coordinator Frank Nilsen Coordinator email frank.nilsen@imr.no
Coordinator institution
IMR - Institute of Marine Research (Norway)
Institutions involved
NA
Start year 2006 End year 2008 Funding (€) € NA Website https://www.fhf.no/prosjekter/prosjektbasen/552312/ Summary The salmon louse is one of the main problems for the salmon farming industry both in Norway and in other countries. Pesticides and different management measures currently control the salmon louse. However, only a few types of drugs are available for salmon louse combat and there are several reports for resistant development/treatment failures for the compounds in use (i.e. emamectin benzoate and pyretroids). This raise the need for new control measures and in this respect vaccination is an attractive strategy if protective antigens can be identified. Recent studies carried out at IMR, shows that significant reduction in lice number can be achieved through vaccination. This study demonstrates that it is possible to control lice using a vaccination strategy. The antigens used in the first test vaccine are part of a biological process likely controlled by ecdysteroids, either directly or indirectly. Hence, characterisation and identification of genes induced by ecdysteroids seems to be of high relevance as candidate genes in a future L. salmonis vaccine. In the present project proposal we aim to identify and test several new lice vaccine antigens and to establish a new tools for screening of candidate antigens. This includes an extension of our 7 K L. salmonis microarray and to use RNAi as an antigen evaluation tool. The new microarray will be extended by ~5,000 new cDNA probes and used to identify genes induced by ecdysteroids (as measured by the transcription of the ecdysteroid receptor (EcR). RNAi will be established and used as a first line-screening tool. As a final step in antigen evaluation antigens selected based on RNAi results (either directly purified or recombinant) will be tested by immunisation of Atlantic salmon and subsequent challenge with L. salmonis. This includes the EcR, which we have identified in our EST database.
Keywords
Fish;
Vaccines development;
Genetic;
Fish health;
Salmon;
Engineering;
Parasite;
Marine Region
76
Not associated to marine areas
0
Marine Region Map
