Acronym NA
Category
Aquaculture
Title Virulens and survival of Vibrio anguillarum in fish Programme National Programme
Instrument (FP6)
Contact Type (FP7)
Strand (Interreg)
NA
Theme (FP7)
Activity Area (FP6)
Regional Area (Interreg)
Action (COST)
NA
Specific Programme (FP7)
NA
Funding source National Coordinator Debra Milton Coordinator email debra.milton@molbiol.umu.se
Coordinator institution
UMU - UMEA University (Sweden)
Institutions involved
NA
Start year 2004 End year 2007 Funding (€) € 8,100 Website NA Summary Disease control is a critical problem in the fish-farming industry. Vibriosis due to Vibrio anguillarum is a serious bacterial disease causing economical losses in aquaculture worldwide. Growth in a multicellular community, such as a biofilm, protects bacteria and aids colonization of fish. Exopolysaccharides surround bacteria in a biofilm and aid invasion of fish by V. anguillarum suggesting that biofilm formation is a mechanism of virulence. We will define biofilm formation on the surface of the fish and determine if a biofilm protects bacteria from the innate immunity of surface mucus. V. anguillarum uses the intestines as a site of attachment, colonization and growth making resistance to bile a vital means of survival in the fish. We have identified a novel outer membrane protein that is needed for bile resistance and expressed primarily in the fish. We will study the expression of this protein and characterize the regulatory proteins needed for its expression in the fish. Therapeutic agents that block biofilm formation or function of new regulatory proteins expressed in the fish may aid disease control.
This study analyses new means of survival and/or virulence for V. anguillarum. Goals of this project are: (1) To characterize a 37-kDa outer membrane protein involved in bile resistance; (2) To determine what genes regulate the expression of the 37-kDa OMP in the presence of bile; (3) To determine what other genes are regulated by these newly identified regulatory proteins; (4) To determine if Vibrio anguillarum forms a biofilm on the surface of the fish and what proteins are needed for evasion of the fish innate immune systems to colonize fish skin tissue.
Keywords
Disease;
Biology;
Genetic;
Fish;
Protein;
Bacteria;
Marine Region
76
Not associated to marine areas
0
Marine Region Map
