Acronym NA
Category
Fisheries
Title Development of a cardiovascular challenge model facilitating functional feeds against viral diseases Programme National Programme
Instrument (FP6)
Contact Type (FP7)
Strand (Interreg)
NA
Theme (FP7)
Activity Area (FP6)
Regional Area (Interreg)
Action (COST)
NA
Specific Programme (FP7)
NA
Funding source National Coordinator Trygve Sigholt Coordinator email NA
Coordinator institution
NA
Institutions involved
NA
Start year 2015 End year 2017 Funding (€) € 630,530 Website https://prosjektbanken.forskningsradet.no/project/FORISS/245396?Kilde=FORISS&distribution=Ar&chart=bar&calcType=funding&Sprak=no&sortBy=date&sortOrder=desc&resultCount=30&offset=30&ProgAkt.3=HAVBRUKS-Havbruk+-+en+n%C3%A6ring+i+vekst Summary "Heart and skeletal muscle inflammation (HSMB) and cardiomyopathy syndrome (CMS, also called heart crack) are two viral diseases that are a growing problem for salmon in the Norwegian farming industry. There are still no vaccines against these diseases. For pancreatic disease (PD) is a vaccine introduced, but with limited effect. The disease is still a major problem for the industry. These three viral diseases affect the heart in addition to other organs. This documentation is expensive and time-consuming to obtain, and have therefore been used to a limited extent. Both lab and field experiments are often very uncertain and do not always have the necessary sensitivity and reproducibility to reveal the effects nutrition has on health. In the project, we wanted to develop a biological fish model to test the effects of nutrition on heart function and circulation in fish. Our results from testing the model showed that it was not precise enough to simulate tissue damage in the heart after an infection to distinguish between different feeds. An alternative model of cultivating immune cells to investigate basic mechanisms behind feed effects was tried with promising results, but will require further development to have practical application. An infection trial with PD, which was carried out to verify the fish model, showed that feed with krill meal gave significantly better growth 10 weeks after infection. Histopathological and gene analyzes (microarray) of the heart showed that feed with krill meal produced less severe PD symptoms and faster rehabilitation. The positive effect on growth of krill meal in the feed was much clearer in infected than uninfected fish. The project ended with a test of two feed concepts based on low and high levels of krill meal in an experiment with salmon from approx. 1 to 4.5 kg in BioMar's experimental concession at Dønna. The aim of the experiment was to test effects on heart health, colour, fillet yield, meat quality and melanin spots in the fillet. Large-scale field trials are always subject to unexpected events and uncontrolled effects. Using only two cages per feed code also limits statistically valid conclusions. There were too few melanin spots in the fillet for it to be possible to find any effect of feed in the experiment. The groups fed krill meal had a darker skin colour. The feed with the most krill meal had a slightly better fillet yield and red color than the control group. This feed also had significantly the most normally triangular-lined hearts and no round (malformed) hearts, indicating a positive effect on heart health. Histopathology and gene expression (qPCR and microarray) showed that the fish were infected with HSMB and CMS. The most obvious infection was from the PMCV virus that causes CMS.PMCV was found in significantly fewer fish in the two groups fed krill meal than in the control group. The difference was not significant, but is nevertheless promising and will be followed up in new trials. Krill meal had an effect on gene expression in the gills and particularly on genes linked to virus defence. This corresponds to histology data and is important documentation for a new feed that can help to strengthen both heart and gill health.
Keywords
Shellfish;
Fish;
Crustacean;
Disease;
Animal feed;
Feed composition;
Krill;
Bioprospecting;
Salmon;
Marine Region
76
Not associated to marine areas
0
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