Acronym TripGenWelfare
Category
Aquaculture
Title The influence of inbreeding and aberrant inheritance on welfare in gynogenetic, triploid, and tetraploid Atlantic salmon Programme National Programme
Instrument (FP6)
Contact Type (FP7)
Strand (Interreg)
NA
Theme (FP7)
Activity Area (FP6)
Regional Area (Interreg)
Action (COST)
NA
Specific Programme (FP7)
NA
Funding source National Coordinator Alison Harvey Coordinator email alison.harvey@hi.no
Coordinator institution
IMR - Institute of Marine Research (Norway)
Institutions involved
NA
Start year 2021 End year 2025 Funding (€) € 756,100 Website https://prosjektbanken.forskningsradet.no/en/project/FORISS/325320?Kilde=FORISS&distribution=Ar&chart=bar&calcType=funding&Sprak=no&sortBy=date&sortOrder=desc&resultCount=30&offset=0&TemaEmne.2=Marin+bioteknologi&source=FORISS&projectId=301535 Summary Escapees are a major challenge to sustainable Atlantic salmon aquaculture, and sterile triploid salmon are a solution. Triploid salmon are produced by a pressure shock and contain two copies of maternal and one copy of paternal DNA. The use of triploids commercially is limited by poor welfare and performance. We hypothesize that doubling of the dam genome may cause an inbreeding effect in the offspring, that, dependent on the heterozygosity of the dam, could explain the lower performance and welfare observed. We will examine the potential inbreeding effect using controlled experiments comparing diploid, pressure-induced triploid and gynogenetic (inbred) diploid full siblings. Gynogenes are diploid offspring containing two copies of only the maternal DNA. We will follow the different groups, assessing fish for performance and welfare. We will use a mixture of genomic and genetic methods to assess the level of inbreeding across the entire genome in each group and compare this to individual performance, which has never been done in triploid Atlantic salmon. We also hypothesize that a sub-optimal protocol may lead to unintended effects on triploids as hydrostatic pressure treatment is known to cause chromosome aberrations. To examine the nature and prevalence of chromosome aberrations resulting from pressure-shock treatment we will assess the incidence and types of errors and how they influence triploid performance/welfare by linking individual performance in controlled experiments to observed chromosome aberrations using genetic tools. An alternative method to produce triploid salmon is by producing tetraploid individuals and breeding them with standard diploids to produce triploids with higher levels of heterozygosity compared to traditionally produced triploids. This has never been done in Atlantic salmon; therefore our final aim is to produce the world´s first Atlantic salmon tetraploid line with the goal of producing triploids by crossing them with diploids.
Keywords
Escapes;
Salmon;
Genetic;
Engineering;
Fish;
Marine Region
76
Not associated to marine areas
0
Marine Region Map
