Acronym MARSENSE
Category
Marine Biotechnology
Title Development of an electrochemical biosensor for monitoring organic contaminants in the marine environment Programme National Programme
Instrument (FP6)
Contact Type (FP7)
Strand (Interreg)
NA
Theme (FP7)
Activity Area (FP6)
Regional Area (Interreg)
Action (COST)
NA
Specific Programme (FP7)
NA
Funding source National Coordinator Teresa Rocha Coordinator email NA
Coordinator institution
CESAM - Centre for Environmental and Marine Studies (Portugal)
Institutions involved
NA
Start year 2018 End year 2022 Funding (€) € 239,015 Website https://www.cesam-la.pt/projetos/marsense-desenvolvimento-de-um-biosensor-eletroquimico-para-monitorizacao-de-contaminantes-organicos-no-ambiente-marinho/ Summary "Various types of pollutants released into the environment by anthropogenic or natural sources can affect seawater quality. For example, persistent organic compounds such as endocrine disruptors and pesticides, and toxins from toxic algae can cause damage to the aquatic environment with effects such as acute and chronic toxicity to aquatic organisms, accumulation in the ecosystem, and losses of habitats and biodiversity. The use of techniques such as HPLC or GC-MS have contributed to the identification and quantification of pollutants in seawater. However, these analytical techniques require sample preparation and sometimes pre-concentration steps in order to carry out quantitative assessment of trace concentrations of contaminants in seawater generally involving long analysis times, costs and resources. . On the other hand, little work has been carried out related to the use of biosensors for the detection of organic contaminants in seawater samples. Therefore, it is necessary to develop detection systems such as biosensors that allow in situ monitoring of contaminants and alert to potential contamination of the marine environment.
This project aims to develop a biosensor based on electrochemical principles for monitoring organic contaminants in seawater. In a first stage, the electrochemical biosensors will be assembled, tested and optimized. Microelectrodes will be constructed, and antibody adsorption and immobilization studies will be carried out. The biosensor will be tested for signal stability using standard solutions. In a second stage, the sensor will be validated under laboratory conditions, that is, the detection of organic contaminants will be done with real samples of seawater collected on the coast and water collected in aquaculture. The results will be compared between the data obtained with the biosensor and those obtained with analytical techniques, such as ELISA and/or GC-MS. The biosensor will also be validated and compared with such analytical techniques in analytical recovery studies. Thirdly, the biosensor will be adjusted to a prototype and tested with real samples, being placed in buoys that will be inserted in aquacultures and on the coast, in order to demonstrate its functionality for in situ monitoring of contaminants. The biosensor could also be used in other situations where warning systems are needed to assess water contamination."
Keywords
Toxic substances;
Algal toxins;
Biosensors;
Monitoring;
Marine Region
38
Portuguese Waters (27.IXa,27.IXb)
1
Marine Region Map
