Acronym NA
Category
Aquaculture
Title Saccus vasculosus, neuroglobin, stress and welfare during slaughter of salmon Programme National Programme
Instrument (FP6)
Contact Type (FP7)
Strand (Interreg)
NA
Theme (FP7)
Activity Area (FP6)
Regional Area (Interreg)
Action (COST)
NA
Specific Programme (FP7)
NA
Funding source National Coordinator Bjørn Olav Kvamme Coordinator email bjornok@imr.no
Coordinator institution
IMR - Institute of Marine Research (Norway)
Institutions involved
NA
Start year 2009 End year 2012 Funding (€) € 584,380 Website https://prosjektbanken.forskningsradet.no/en/project/FORISS/190021?Kilde=FORISS&distribution=Ar&chart=bar&calcType=funding&Sprak=no&sortBy=date&sortOrder=desc&resultCount=30&offset=180&Departement=Fiskeri-+og+kystdepartementet&source=EU&projectId=775998 Summary Slaughter of fish is not optimal from a welfare point of view. We lack understanding of the oxygen storage protein neuroglobin in the fish brain and the hemered proteins in the Saccus vasculosus. Present methods used to stun and slaughter fish: electricity, percussion, chilling and carbon dioxide (CO2) are stressful. Sedation followed by anaesthetisation and slaughter that does not evoke stress responses would be very beneficial. We have found that CO has sedation and anaesthetic effects in fish. We believe that CO binds to oxygen storage proteins in the Saccus vasculosus and neuroglobin in the brain and cause sedation and anaesthetisation of fish/animals. From a welfare point this is very favourable, and the aim is to gain basic knowledge on how hemered proteins in the Saccus vasculosus and neuroglobin function as an O2 storage component in brain and how stress regulates transcription and translation of Atlantic salmon neuroglobin. The hemered proteins of the Saccus vasculosus will be quantified, and neuroglobin will be isolated and characterized using classical analytical methods. Experiments using hypoxic conditions have shown that the amount of globins mRNAs change in salmon. The sequence of both neuroglobin and its genetic origin in salmon will be determined. Spectra of extracts from the Saccus vasculosus and gel chromatography indicate the presence of a high molecular haemoglobin component and a low molecular myoglobin component that is likely to be neuroglobin. CO will be used for sedation, anaesthetisation and slaughtering of salmon. Animal welfare, i.e. stress, will be measured using cortisol, brain and heart waves and behaviour responses. CO binds to the heme group of proteins and oxidation and rancidity are reduced. CO functions as a bacteriostatic agent, improving storage properties of fish fillets. Red colour of the product, due to CO binding, promotes fish and meat products in the market.
Keywords
Animal welfare;
Genetic;
Salmon;
Process efficiency;
Fish;
Slaughtering systems ;
Engineering;
Marine Region
76
Not associated to marine areas
0
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