Acronym NA
Category
Fisheries
Title Hurtig metode for å estimere buksprenging i pelagisk fisk ombord - Quick method for estimating abdominal rupture in pelagic fish on board Programme National Programme
Instrument (FP6)
Contact Type (FP7)
Strand (Interreg)
NA
Theme (FP7)
Activity Area (FP6)
Regional Area (Interreg)
Action (COST)
NA
Specific Programme (FP7)
NA
Funding source National Coordinator Rasa Slizyte´ Coordinator email Rasa.Slizyte@sintef.no
Coordinator institution
SINTEF-SFH - SINTEF Fisheries and Aquaculture (Norway)
Institutions involved
NA - SINTEF Ocean (Norway) ,
CSIC-IIM - Spanish National Research Council;Institute of Marine Research (Spain) ,
Start year 2011 End year 2013 Funding (€) € 179,080 Website https://www.fhf.no/prosjekter/prosjektbasen/900656/ Summary Today, the fleet is entitled to report the amount of bait in the catch on a subjective scale (1-4) which does not give a correct picture of the danger of abdominal rupture. Therefore, and following the fishermen's desire to find an objective method for predicting trout blasting, SINTEF Fisheries and Aquaculture (FH) has worked for a number of years with the bait and trout blasting problem in pelagic catches. As a result of several projects and a doctoral dissertation, the enzymatic activities that leak from the fish's digestive system and cause the explosion have been identified. Furthermore, we tested two promising variants of assays that would identify the presence of proteolytic activity: (1) Petri dishes with gelatin: The activities found in the ventral muscle are found to break down gelatin. If one abdominal muscle extract breaks down gelatin, that is, strong proteases are present, indicating leakage of digestive enzymes since the muscle itself does not contain such strong protein-degrading enzymes, (2) ATP luminometer: Measures the amount of ATP in a sample using luciferase, which produces 1 photon (1 light unit) per ATP molecule. If proteases are present in the sample, these will be able to break down the luciferase and therefore inhibit light production.
Goals:
To test and adapt a quick and easy measurement method to estimate the belly burst of pelagic catch for use in boats and in industry.
Sub-goal
1. To complete the development protocol for use on board fishing boats and in the land industry.
2. To test with and without bait on freshly caught fish (including cruise activity).
3. To optimize the measurement method, training of fishermen and proposals for implementation.
Keywords
Fish;
Fish quality;
Marine Region
41
Norwegian Sea (27.IIa)
13
Northern North Sea (27.IVa)
14
Skagerrak, Kattegat (27.IIIa)
42
Barents Sea (27.I)
4
Marine Region Map
