Acronym NA
Category
Fisheries
Title Species identification of Icelandic marine organisms using a genetic analyses technique Programme National Programme
Instrument (FP6)
Contact Type (FP7)
Strand (Interreg)
NA
Theme (FP7)
Activity Area (FP6)
Regional Area (Interreg)
Action (COST)
NA
Specific Programme (FP7)
NA
Funding source National Coordinator Sigurlaug Skírnisdóttir Coordinator email sigurlaug.skirnisdottir@matis.is
Coordinator institution
MATIS - Matis Ltd (Iceland)
Institutions involved
NA
Start year 2007 End year 2009 Funding (€) € NA Website https://matis.is/skyrsla/throun-erfdagreiningaradferdar-til-tegundaakvordunar-helstu-nytjastofna-islands-species-identification-of-icelandic-marine-organisms-using-a-genetic-analyzes-technique/ Summary The goal of the project was to develop a sequencing database for three chronometer mitochondria genes for 26 Icelandic marine species. Furthermore, to develop a DNA protocol to analyze mixed unknown samples, such as juveniles of fish and identification of fish in fish stores. Classical morphological identification of marine species is time‐consuming and depends on a high degree of taxonomic expertise. This expertise is currently falling short, therefore, in many cases the identification of a species is the major bottleneck in marine biodiversity and ecosystem research. On demand, molecular diagnostic techniques have proven to be successful species identification tools. Recently, DNA barcoding has been highly accepted as a rapid, cost-effective and widely applicable tool for species identification. Currently, the three most common DNA barcoding targets are the mitochondrial genes cytochrome c oxidase I (COI), cytochrome b (Cytb) and 16S RNA (16S). For DNA barcoding, the mtDNA gene, cytochrome c oxidase I (COI), has been highlighted as the genetic marker for species identification in huge international projects, like the project "Barcode of Life". In this project we did a partial sequencing of these three genes of 26 Icelandic marine organisms that are used in Iceland. The method is straight forward; DNA is isolated from the specimen, the three genes are PCR amplified in separate reactions using universal primers and then sequenced. A database was developed, saving the sequences obtained in the project for the three genes. Finally, 24 unknown samples (blind samples) were analyzed. Part of the samples were fish fillets from fish shops, some were samples from the databank of MRI and some samples were juvenile fish that were difficult to identify by morphology. All samples were species identified easily by using the sequencing method, supporting the importance of the method. DNA species identification is more rapid, cost-effective and more accurate than the classical morphological identification method. Therefore, this method is an important tool for the industry to ensure reliable identification of marine organisms in all life stages and process stages.
Keywords
Genetic;
Fish;
Genomic sequencing;
Monitoring;
Protocol;
Marine Region
36
Iceland Grounds (27.Va)
1
Marine Region Map
