Acronym NA
Category
Seafood Processing
Title Optimization of HPLC method for determination of histamine and other biogenic amine in seafood Programme National Programme
Instrument (FP6)
Contact Type (FP7)
Strand (Interreg)
NA
Theme (FP7)
Activity Area (FP6)
Regional Area (Interreg)
Action (COST)
NA
Specific Programme (FP7)
NA
Funding source National Coordinator Vida Simat Coordinator email vida@unist.hr
Coordinator institution
UNIST - University of Split (Croatia)
Institutions involved
DTU-FOOD - Technical University of Denmark; National Food Institute (Denmark) ,
Start year 2009 End year 2009 Funding (€) € 333,333 Website NA Summary Between January 2004 and June 2007. Technical University of Denmark studied Biogenic amines in seafood -assessment and management of consumer exposure within the FP6 BIOCOM project with Paw Dalgaard as project leader. Project has collected information about the occurrence of histamine fish poisoning (HEP) worldwide. The implicated fish species and products were detected, as well as key concentrations of histamine implicated in HEP and bacteria responsible for histamine formation. The project gave serious reflecting on fish manipulation parameters. To change the present state of HEP optimization of existing chromatographic methods is required. New HPLC column material with smaller particle size is becoming available which allows existing chromatographic methods to be optimized so that a similar analytical performance is obtained with smaller volumes of reagents and thereby cheaper analyses, resulting with a faster and more accessible method with ranging usage in defining critical control points in fish manipulation methods and quality control. During my research visit comparison of HPLC column material with of 2, 3 and 5 micra will be compared and chromatographic conditions optimized for determination of biogenic amine in seafood. In addition extractions procedures will be evaluated and the developed HPLC method will be compared quantitatively with available rapid test kits for determination of histamine in seafood. Results should affirm the most effective particle sizes of HPLC column material for rational method usage. Fish extractions procedures with also acquire additional customization to a new analytical HPLC method. Developed HPLC method will be compared quantitatively with available rapid test kits for determination of histamine in seafood. The expected result after three month research visit is new, optimized, accurate and accessible HPLC method that will make a contribution to scientific research, as well as fish industry laboratories. A unique method will provide easier data analysis from different subjects, which will provide in faster detection and control management of biogenic amine formation in seafood.
The objective of the project was to modify HPLC methods to use smaller sized column particles of 1.8e3 mm and to compare their performance with corresponding classical HPLC methods using column particles of 5 mm. The methods were compared with respect to the time of elution, eluent consumption, separation of biogenic amines as well as recovery and repeatability for determination of biogenic amines in lean canned tuna and fatty frozen herring.
Keywords
Herring;
Fish;
Seafood;
Tuna;
Technology;
Food safety;
Marine Region
76
Not associated to marine areas
0
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