Acronym PARRTAE
Category
Marine Biotechnology
Title Probing Antibiotic Residues and Resistance transfer in Aquatic Environments Programme International Cooperation
Instrument (FP6)
Contact Type (FP7)
Strand (Interreg)
NA
Theme (FP7)
Activity Area (FP6)
Regional Area (Interreg)
Action (COST)
NA
Specific Programme (FP7)
NA
Funding source National-European Coordinator Asa Sjöling Coordinator email asa.sjoling@ki.se
Coordinator institution
KI - Karolinska Institutet (Sweden)
Institutions involved
EV ILVO - Flanders Research Institute for Agriculture, Fisheries and Food (Belgium) ,
NTNU - Norwegian University of Science and Technology (Norway) ,
SLU - Swedish University of Agricultural Sciences (Sweden) ,
ULPGC - University of Las Palmas de Gran Canaria (Spain) ,
Start year 2021 End year 2024 Funding (€) € NA Website https://www.era-learn.eu/network-information/networks/aquaticpollutants/1st-joint-call-2020/probing-antibiotic-residues-and-resistance-transfer-in-aquatic-environments Summary The project aims to study bacteria, antibiotic resistance genes (ARGs) and antibiotic residues in groundwater, surface water, marine water environments in the North Sea and Atlantic including ports and aquaculture facilities.
Samples will be collected from sites with high and low suspected loads of antibiotic residues. Culture-based and sequencing-based methods will be used to identify ARGs.
Transfer of ARG-containing plasmids will be analysed using indicator bacteria Escherichia coli, Vibrio spp, and Shewanella algae as recipients. LC-MS/MS will be used to quantify the levels of different antibiotics in the collected samples. We hypothezise
that local water microbiota, antibiotic residues and recipient species will affect the type of plasmids transferred.
The effects of the acquired plasmids on the physiology of our bacterial models will be analysed in experiments lab systems, and their influence on fitness and virulence in a live host will be studied by host interactions in a shrimp (Artemisia) model.
The project will determine common ARG plasmids circulating in European waters and their inherent properties as a fundament to understand and prevent their dissemination.
Keywords
Antibacterial;
Bacteria;
Genomic sequencing;
Toxic substances;
Human health;
Metagenomic;
Monitoring;
Marine Region
5
Southern North Sea (27.IVc)
6
Central North Sea (27.IVb)
13
Northern North Sea (27.IVa)
2
Canarias and Madeira Islands (34.1.2)
4
Marine Region Map
