Acronym NA
Category
Marine Biotechnology
Title Development and transfer of technology for the creation of a genetic sperm bank from genetically selected breeders from gilthead seabream Programme National Programme
Instrument (FP6)
Contact Type (FP7)
Strand (Interreg)
NA
Theme (FP7)
Activity Area (FP6)
Regional Area (Interreg)
Action (COST)
NA
Specific Programme (FP7)
NA
Funding source National Coordinator Gonzalo Martinez Rodriguez Coordinator email gonzalo.martinez@icman.csic.es
Coordinator institution
CSIC - Spanish National Research Council (Spain)
Institutions involved
NA - Maresa (Spain) ,
MINECO - Ministry of Economy and Competitiveness (Spain) ,
ULE - University of Leon (Spain) ,
Start year 2006 End year 2008 Funding (€) € 78,651 Website NA Summary Aquaculture has been a technological breakthrough in recent years that significant efforts are being devoted to gene characterization of stocks and the development of breeding programs. These programs require programming junctions and necessarily include the continued availability of gametes. In this paper we propose to: (1) Bring industrial scale techniques for sperm cryopreservation of gilthead seabream, so as to ensure the ability to maintain indefinitely keeping intact sperm quality; (2) Check that the damage caused to the chromatin during the freezing process does not affect the genetic variability of offspring; (3) To transfer the techniques to the enterprise so it can develop its own sperm bank. The use of gilthead seabream is of special interest because this species of great commercial value, is a protandrous species, so that individuals have a life too short fertile as males. In addition, the teams have been previous studies that have begun to set-freezing techniques laboratory scale with very satisfactory results and have microsatellite and AFLP markers very useful for the development of the project. The first stage will be an assessment and characterization of semen quality. Different parameters were determined normal semen quality (motility, pH, osmolarity, fertilizing capacity), and different factors that can affect sample freezability (mitochondrial activity, integrity and plasma membrane composition, etc.). Subsequently tested different methods of cryopreservation, thawed samples examined for changes in the composition and function of plasma membrane (fusogenicity, regionalization, etc.), and chromatin alterations (Comment assay). Those diluents and freezing methods best-performing small scale will be adapted for use in larger volumes (up to 5ml), putting special emphasis on the development of media suitable for use fertilization with thawed semen. The correlation study of the different data obtained allow us to choose the most appropriate method of cryopreservation and establish criteria for the selection of potentially suitable semen samples to be cryopreserved. This phase of the project will be coordinated by the team of the ULE. Subsequently, in order to assess the consequences of the damage that can occur during freezing chromatin, ICMAN team shall study the genetic variability of the offspring. Will be programmed crosses between males and females used previously characterized fresh and frozen semen. Type will be used microsatellites and AFLP markers. To transfer to the company will advise on the acquisition and management of equipment needed and one of the team members ULE make a stay in the company in order to teach and train the technicians of the company in evaluating seminal and the freezing process and handling of cryopreserved samples. To facilitate coordination, as well as trade and movements requiring experimental development will be two joint meetings. The main objective of the project is therefore to develop the technology that allows indefinitely preserving sperm, ensuring the conservation of all its genetic potential, as a tool for managing reproduction and developing selection programs. This objective is broken down into two consecutive goals: (1) Adaptation of cryopreservation methods to the freezing of gilthead sea bream sperm in 2ml vials and 5ml macrotubes. This goal aims to design a freezing method that guarantees maximum post-thaw fertility and minimizes damage to the structure and genome of spermatozoa, in order to prevent loss of genetic potential; (2) Transfer of the technology to the company (facility adaptation and personnel training).
Keywords
Technology;
Fish;
Fish reproduction;
Seabream;
Marine Region
76
Not associated to marine areas
0
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