Acronym NA
Category
Marine Biotechnology
Title Development of novel and improvement of current methods for the diagnosis of Koi Herpesvirus (KHV) as well as development and implementation of an effective vaccine Programme National Programme
Instrument (FP6)
Contact Type (FP7)
Strand (Interreg)
NA
Theme (FP7)
Activity Area (FP6)
Regional Area (Interreg)
Action (COST)
NA
Specific Programme (FP7)
NA
Funding source National Coordinator NA Coordinator email NA
Coordinator institution
TiHo - University of Veterinary Medicine Hannover (Germany)
Institutions involved
NA - Fraunhofer-Gesellschaft (Germany) ,
FAU - Friedrich Alexander University Erlangen-Nuremberg (Germany) ,
Start year 2015 End year 2019 Funding (€) € 1,020,637 Website https://service.ble.de/ptdb/index2.php?detail_id=57200&site_key=141&stichw=Kryokonservierung+Karpfen&zeilenzahl_zaehler=17 Summary Pond aquaculture of carp provides a supply of fish products in high quality to consumers from a local and sustainable production. In addition, in some areas of Germany, carp farms are main components of the traditional landscape, are having great importance for water budget and wildlife habitat and offering qualified places of employment in less structured areas. In several parts of Germany, carp farming is severely affected by Koi Herpesvirus Disease (KHVD), caused by the infection of carp with the Cyprinid herpesvirus 3 (CyHV-3), also known as Koi Herpesvirus, KHV). Outbreaks of the disease may cause severe economic losses due to associated mortality of infected carp, costs for destruction of affected fish, disinfection of affected ponds and subsequent absence of earnings from carp production. This situation severely challenges the economic basis of affected farms and may result in closing down the operation. Currently no measures for prevention or cure of this disease are available in order to reduce the economic losses for the farmers. Thus, valid and effective tools for monitoring and controlling of this virus disease and effective combat against the virus are needed. The main obstacles are: • Available diagnostic procedures relay on the detection of virus DNA and do not allow recognizing the presence of infective virus particles; • Effective vaccines for a reduction of losses and a prevention of further distribution of the infection are lacking; Aim of the proposed study: In order to develop strategies to contain the disease from endemic areas and to protect naive carp populations in virus free areas, an improvement of diagnostic procedures and preventive measures are needed. Therefore, we aim at: • Improvement of the diagnostics of a CyHV-3 infection by means of cell culture systems • Development of new vaccination concepts including new adjuvants, which effectively prevent losses and would allow recognizing vaccinated populations from latently / persistent For this purpose, we are planning to • Develop new cell culture systems from various cell types obtained from carp or other fish, with high proliferation capacities, like endothelia or adult stem-/ progenitor cells and compare their suitability for CyHV-3 replication and their effectiveness to produce high titers of the virus. • Standardize and optimize cell cultivation protocols for isolation and replication of the virus in newly developed and already established permanent or long term cell lines by determining optimal cultivation conditions for virus replication as a basis for reliable diagnostic and a virus replication sufficient for an economic vaccine production. • Raise an effective vaccine based on a virus which was attenuated by controlled induction of mutations and which includes natural products like host defensins or algal products as adjuvants as a protection of carp from this devastating disease.
Keywords
Diagnostic application;
Carp;
Disease;
Vaccines development;
Fish;
Fish health;
Land-based aquaculture;
Monitoring;
Marine Region
76
Not associated to marine areas
0
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