Acronym NA
Category
Marine Biotechnology
Title Papel del GDF-9 y BMP-15 en la regulación de las fases iniciales del desarrollo ovárico en peces y su relación con la fertilidad: Estudio de aplicaciones potenciales para el control de la reproducción - Role of GDF-9 and BMP-15 in the regulation of the initial phases of ovarian development in fish and its relationship with fertility: Study of potential applications for the control of reproduction Programme National Programme
Instrument (FP6)
Contact Type (FP7)
Strand (Interreg)
NA
Theme (FP7)
Activity Area (FP6)
Regional Area (Interreg)
Action (COST)
NA
Specific Programme (FP7)
NA
Funding source National Coordinator Francisco Prat Baella Coordinator email f.prat@csic.es
Coordinator institution
CSIC-ICMAN - Spanish National Research Council; Institute of Marine Science of Andalucía (Spain)
Institutions involved
INIA - National Institute of Agricultural and Food Research and Technology (Spain) ,
Start year 2007 End year 2010 Funding (€) € 133,100 Website https://www.observatorio-acuicultura.es/recursos/bases-de-datos/proyectos/papel-del-gdf-9-y-bmp-15-en-la-regulacion-de-las-fases-iniciales Summary During oogenesis in fish, a great number of oocytes develop to reach final maturity. The recruitment of these oocytes at the beginning of the ovarian cycle, inducing them to grow, is of vital importance for determining the number of eggs that will finally be ovulated. There is a lack of information concerning how the early stages of oocyte development are controlled and which factors can be involved. Very recently, we have cloned two growth factors, the growth differentiation factor 9 (GDF-9) and the bone morphogenetic protein 15 (BMP-15) in the European sea bass. In mammals, these two factors are essential for a normal oocyte growth and his malfunction provokes infertility. The preliminary results that we have obtained in sea bass suggest a similar function in fish since these genes are expressed early in the reproductive cycle. If our hypothesis were true, these genes would be good markers for infertility in fish, and it would also allowed us to study the development of techniques to artificially control ovarian growth. Therefore, obtaining information about the process of egg recruitment in fish would be of great advantage for optimising fish aquaculture. In this project we propose: (1) To develop molecular and immunological tools for the study of GDF-9 and BMP-15 function during ovarian development; (2) To determine the cellular localization of GDF-9 and BMP-15 mRNAs by in situ hybridization and the resultant proteins by immunocytochemistry; (3) To determine the GDF-9 and BMP-15 ability to stimulate ovarian development and determine their function on the regulation of early ovarian growth; (4) To determine the relation of GDF-9 and BMP-15 with fertility in fish and study potential applications for the control of reproduction.
(1) To develop molecular and immunological tools for the study of GDF-9 and BMP-15 function during ovarian development; (2) To determine the cellular localization of GDF-9 and BMP-15 mRNAs by in situ hybridization and the resultant proteins by immunocytochemistry; (3) To determine the GDF-9 and BMP-15 ability to stimulate ovarian development and determine their function on the regulation of early ovarian growth; (4) To determine the relation of GDF-9 and BMP-15 with fertility in fish and study potential applications for the control of reproduction.
Keywords
Life cycle;
Genetic;
Seabass;
Fish biology;
Fish reproduction;
Fish;
Marine Region
76
Not associated to marine areas
0
Marine Region Map
