Acronym NA
Category
Marine Biotechnology
Title Multiple routes to B cell memory in Atlantic salmon Programme National Programme
Instrument (FP6)
Contact Type (FP7)
Strand (Interreg)
NA
Theme (FP7)
Activity Area (FP6)
Regional Area (Interreg)
Action (COST)
NA
Specific Programme (FP7)
NA
Funding source National Coordinator Jorunn B. Jørgensen Coordinator email jorunn.jorgensen@uit.no
Coordinator institution
UiT - The Arctic University of Norway (Norway)
Institutions involved
NA
Start year 2016 End year 2022 Funding (€) € 833,300 Website https://prosjektbanken.forskningsradet.no/en/project/FORISS/254892?Kilde=FORISS&distribution=Ar&chart=bar&calcType=funding&Sprak=no&sortBy=date&sortOrder=desc&resultCount=30&offset=180&ProgAkt.3=HAVBRUK2-Stort%20program%20for%20havbruksforskning&source=FORISS&projectId=196649 Summary Viral diseases like pancreas disease are a main concern in the Norwegian aquaculture industry, and the vaccines on the market give suboptimal protection. B cell memory responses are essential for durable protective immunity after vaccination. The organization of the teleost immune system and its B cell components differ from mammals, and it is likely that these features influence how immunological memory is maintained. It is therefore a need to determine the protective status of vaccines related to elicited B cell responses. However, the components and mechanisms underlying formation of and function of A. salmon B cell memory are still elusive. In this project we propose to study salmon IgM+ and IgT+ B cells, their distribution, and the quantity and quality of B cell memory responses after vaccination. The main question is how and to what extend salmon preserve B cell memory. Our own previous work has shown that a high and long lasting antibody response is induced in vivo using the TLR ligands (CpG/poly I:C) as adjuvants in combination with salmonid alphavirus (SAV) antigen. The antibody levels were significantly higher than with other adjuvants, suggesting that TLR stimulation mediate salmon B cell effector functions. This model will be used as a basis in the proposed project to explore how TLR ligands influence B cell memory and whether B cells of memory and/or plasma cell phenotypes exist in A.salmon. To identify different B cell subpopulations and explore their functions a wide repertoire of state of the art techniques will be employed including proteomics, flow cytometry and RNAseq. If successful, our research will guide the design of future vaccines and also provide a set of valuable tools to measure salmon B cell levels and functions.
Keywords
Salmon;
Fish;
Fish health;
Fish biology;
Disease;
Marine Region
76
Not associated to marine areas
0
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