Acronym NA
Category
Aquaculture
Title Steril laks: Kartlegging av sentrale faktorer i dannelsen av kjønnsceller hos Atlantisk laks - Sterile salmon: Mapping of key factors in the formation of gametes in Atlantic salmon Programme National Programme
Instrument (FP6)
Contact Type (FP7)
Strand (Interreg)
NA
Theme (FP7)
Activity Area (FP6)
Regional Area (Interreg)
Action (COST)
NA
Specific Programme (FP7)
NA
Funding source National Coordinator Helge Tveiten Coordinator email helge.tveiten@nofima.no
Coordinator institution
NOFIMA - Norwegian Institute of Food, Fisheries and Aquaculture Research (Norway)
Institutions involved
NA - AquaGen AS (Norway) ,
Start year 2013 End year 2015 Funding (€) € 255,733 Website https://www.fhf.no/prosjekter/prosjektbasen/900791/ Summary The project is a step forward to establish efficient and good methods for the production of sterile salmon without adverse effects on production and fish welfare. The protein Buc, which has a role in the establishment of gametes in salmon, has been thoroughly investigated in the project, and is considered a clear candidate for developing molecular biological and immunological methods for the production of sterile fish. Sexual maturation in aquaculture is considered a problem with regard to escapes and interactions with wild fish, lost growth and reduced fish welfare. In-depth knowledge of specific genes and proteins involved in the formation and development of germ cells / germ cells in fish has now opened up the development of molecular biological and immunological methods for the production of sterile fish. The project has investigated how different genes of crucial importance for the establishment of the gamete cell line are expressed through different phases of ovarian development in salmon. In particular, the Buc protein, which has not previously been characterized in salmon with a tetraploid genome, has been looked at more closely. Four variants of the Buc gene were found in salmon, Buc1a, Buc1b, Buc2a and Buc2b, where Buc1b is a pseudogen that is not expressed. The three active Buc genes were identified as both mRNA and protein in ovulated and newly fertilized eggs. Buc localizes to structures during early cell division (cell division furrow) which indicate that the protein is involved in the establishment of the gamete cell line in salmon. In the early stages of ovarian development, Buc co-locates with several other proteins involved in germ cell development into a structure in the oocyte / egg called the Balbiani body. Based on findings made in the project, Buc is a clear candidate for developing molecular biological and immunological methods for the production of sterile fish.
Keywords
Protein;
Salmon;
Genetic;
Fish biology;
Fish;
Fish reproduction;
Marine Region
76
Not associated to marine areas
0
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