Acronym NA
Category
Marine Biotechnology
Title Production of all-female cod as a means to circumvent problems with early puberty in males: Studies on sex determination and differentiation Programme National Programme
Instrument (FP6)
Contact Type (FP7)
Strand (Interreg)
NA
Theme (FP7)
Activity Area (FP6)
Regional Area (Interreg)
Action (COST)
NA
Specific Programme (FP7)
NA
Funding source National Coordinator Geir Lasse Taranger Coordinator email geirt@imr.no
Coordinator institution
IMR - Institute of Marine Research (Norway)
Institutions involved
NA
Start year 2006 End year 2010 Funding (€) € 380,000 Website https://prosjektbanken.forskningsradet.no/project/FORISS/172633?Kilde=FORISS&distribution=Ar&chart=bar&calcType=projects&Sprak=no&sortBy=score&sortOrder=desc&resultCount=30&offset=30&Fritekst=performance+in+real+sea&source=FORISS&projectId=308843 Summary Early puberty is a major problem in cod farming both due to negative impact on production performance in on-growth farms, and due to risk of negative genetic impact on wild stocks following spawning in sea cages or after escape. Use of all-female stocks can mitigate some of these problems. However, no information is available on the sex determination mechanism in Atlantic cod, the timing of sex differentiation, or the susceptibility to exogenous hormone treatments for inducing sex reversal. Thus the current projects aims to reduce problems connected with early puberty in male cod by developing the basic knowledge required for the production of all-female populations. Specifically, it will attempt to identify the nature of the sex-determining system in cod by: 1) Studying karyology with a complementary range of cytogenetic techniques to determine if the genotypic sex can be recognized directly, and by inducing gynogenetic development to recognize the sex determination system indirectly. 2) Describing the timing of sex differentiation in cod by profiling the expression of genes involved in this process in other vertebrates, such as P450 aromatase, Anti-Müllerian Hormone and Dmrt-1 by quantitative real time PCR and/or by in situ hybridisation, and by histological examination of gonadal sex differentiation including - if required - the quantification of germ cell proliferation via immunohistochemical detection of an endogenous proliferation marker (phosphorylated histone-3). 3) Testing the sex reversing effects of exposure to androgens in putative sensitive time windows, as deducted from the results on sex differentiation, in order to establish optimal timing, dosage, and way of administration of androgens to produce neo-males.
Keywords
Fish biology;
Cod;
Genetic;
Fish reproduction;
Fish;
Marine Region
76
Not associated to marine areas
0
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