Acronym NA
Category
Marine Biotechnology
Title Protection against intracellular pathogens - T-cell based immunity and vaccines Programme National Programme
Instrument (FP6)
Contact Type (FP7)
Strand (Interreg)
NA
Theme (FP7)
Activity Area (FP6)
Regional Area (Interreg)
Action (COST)
NA
Specific Programme (FP7)
NA
Funding source National Coordinator Søren Grove Coordinator email soren.grove@vetinst.no
Coordinator institution
NVI - Norwegian Veterinary Institute (Norway)
Institutions involved
NA
Start year 2012 End year 2017 Funding (€) € 400,000 Website https://prosjektbanken.forskningsradet.no/en/project/FORISS/216154?Kilde=FORISS&distribution=Ar&chart=bar&calcType=funding&Sprak=no&sortBy=date&sortOrder=desc&resultCount=30&offset=60&Organisasjon.3=VETERIN%C3%86RINSTITUTTET Summary Efficacious and safe vaccines are the ideal measure of preventive disease control, also in aquaculture. Such vaccines however, are not available for a number of important fish pathogens and prospects of their development are hampered by lack of information about piscine adaptive immunity and T cells in particular. Vaccination by principle is a manipulative approach and firm knowledge about the object of manipulation - the immune system - is thus of fundamental importance. The present project will characterise the basic biology of T cells in Atlantic salmon and establish parameters for cellular protection, facilitating rational design of vaccines against important viral pathogens. To achieve this, a number of essential reagents and experimental systems will be developed and evaluated, including recombinant proteins, (monoclonal) antibodies and T cell cultures. T cells will subsequently be characterised in substantial detail by advanced molecular methods such as deep-sequencing, DNA chips and single-cell RT -PCR. It is in particular an aim to develop cellular in vitro assays based on multi-channel flow cytometry that allow a functional evaluation of the quality of T cell based immunity. In small-scale experiments, vaccines and vaccine strategies known to be (more) efficacious will be evaluated regarding 'T cell quality' and compared with vaccines having low(er) efficacy. By determining what characterises a protective T cell response to a given pathogen, it will be possible to design vaccines that aim at inducing the same advantageous state.
Keywords
Disease;
Salmon;
Genetic;
Vaccines development;
Fish;
Marine Region
76
Not associated to marine areas
0
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