Acronym NA
Category
Marine Biotechnology
Title Protein-protein interactions between infectious pancras virus proteins and host protein Programme National Programme
Instrument (FP6)
Contact Type (FP7)
Strand (Interreg)
NA
Theme (FP7)
Activity Area (FP6)
Regional Area (Interreg)
Action (COST)
NA
Specific Programme (FP7)
NA
Funding source National Coordinator Jorunn B. Jørgensen Coordinator email jorunn.jorgensen@uit.no
Coordinator institution
UiT - The Arctic University of Norway (Norway)
Institutions involved
NA
Start year 2004 End year 2009 Funding (€) € 720,735 Website https://prosjektbanken.forskningsradet.no/en/project/FORISS/159326?Kilde=FORISS&distribution=Ar&chart=bar&calcType=funding&Sprak=no&sortBy=date&sortOrder=desc&resultCount=30&offset=30&Organisasjon.5=Norges+fiskerih%C3%B8gskole Summary In the post-genomic area new approaches have been developed to study and characterize protein functions in cells and organisms. One such strategy is the yeast two-hybrid system that can be used to generate complete protein interactions maps. Infectious pancreatic necrosis is one o the most threatening diseases in Norwegian aquaculture, and the disease has a worldwide distribution. The causative agent of this disease is infectious pancreatic necrosis virus (IPNV), a prototype virus of the Birnaviridae family. The virus has a double-straned RNA genome divides into two segments and encodes 5 proteins (VP1 to 5). Certainly one of the most important and challenging problems in IPNV biology is defining a host environment and the specific factors, both in host and virus, required for infectious virus production and persistence. The aim of this project is to use the yeast-two hybrid system combined with pull-down experiments to examine potential interactions between all IPNV proteins and in addition interactions between VP3 and viral RNA. Furthermore the yeast two-hybrid screening will be extended to map interactions between IPNV proteins and hos cell defence proteins using a whole library approach. Crystallization of VP2 and VP3 wi ll be attempted for the purpose of resolving their structures by X-ray crystallography.
Keywords
Fish;
Disease;
Genetic;
Marine Region
76
Not associated to marine areas
0
Marine Region Map
