Acronym NA
Category
Marine Biotechnology
Title Studies of virulence mechanisms and host responses to infection with piscine myocarditis virus (PMCV) Programme National Programme
Instrument (FP6)
Contact Type (FP7)
Strand (Interreg)
NA
Theme (FP7)
Activity Area (FP6)
Regional Area (Interreg)
Action (COST)
NA
Specific Programme (FP7)
NA
Funding source National Coordinator Øystein Evensen Coordinator email oystein.evensen@veths.no
Coordinator institution
NMBU - Norwegian University of Life Sciences (Norway)
Institutions involved
NOFIMA - Norwegian Institute of Food, Fisheries and Aquaculture Research (Norway) ,
NVH - Norwegian School of Veterinary Science (Norway) ,
Start year 2012 End year 2016 Funding (€) € 711,000 Website https://prosjektbanken.forskningsradet.no/en/project/FORISS/216177?Kilde=FORISS&distribution=Ar&chart=bar&calcType=funding&Sprak=no&sortBy=date&sortOrder=desc&resultCount=30&offset=0&Organisasjon.5=Institutt%20for%20basalfag%20og%20akvamedisin Summary The aetiology of CMS was defined through the identification of the causative agent, piscine myocarditis virus (PMCV). Built on this new knowledge, we propose a joint research project between the Norwegian School of Veterinary Science and NOFIMA to further explore the pathogenic traits of the virus with an approach to study the detailed infection mechanisms in vitro as well as host immune and immune-related responses to infection plus environmental impact on disease susceptibility. In doing so we will be able to pinpoint risk factors associated with the infection, knowledge that can be used for disease prevention and control in an aquaculture setting. The methodology employed will include reverse genetics with the purpose to obtain a unique tool for in vitro and in vivo studies of infection mechanisms. We will further characterize the virus proteins in terms of immunochemical properties and the viral entry and exit strategies. To obtain a detailed understanding of the virus protein characteristics, the crystal structure of the surface proteins will be elucidated at Ångstrom level through collaboration with Institut Pasteur in France and Dr. Felix Rey. In vivo studies will be performed with the purpose of understanding host responses to infection and particularly the importance of high and low responders in terms of immune responses possibly correlating with severity of heart pathology. Further attempts will be made to pinpoint possible links with high and low stress response patterns in groups of salmon, based on swimming tests. Possible interaction with environmental factors like hypoxia and the interaction between PMCV and another myocarditis virus (piscine reovirus causing HSMI). The results emerging from this project will provide new knowledge of infection mechanisms and host responses to infection and will provide tools for better disease control and prevention.
Keywords
Fish;
Genetic;
Disease;
Salmon;
Marine Region
76
Not associated to marine areas
0
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