Acronym SafeFish
Category
Aquaculture
Title Implications of green fish feed for consumer safety - carry-over of plant peptides, natural toxins and bioactive compounds Programme National Programme
Instrument (FP6)
Contact Type (FP7)
Strand (Interreg)
NA
Theme (FP7)
Activity Area (FP6)
Regional Area (Interreg)
Action (COST)
NA
Specific Programme (FP7)
NA
Funding source National Coordinator Christiane Fæste Coordinator email NA
Coordinator institution
NVI - Norwegian Veterinary Institute (Norway)
Institutions involved
NA - Haukeland University Hospital (Norway) ,
UIN - Nord University (Norway) ,
NOFIMA - Norwegian Institute of Food, Fisheries and Aquaculture Research (Norway) ,
CU Denver - University of Colorado Denver (United States of America) ,
UiO - University of Oslo (Norway) ,
Start year 2016 End year 2020 Funding (€) € 870,500 Website https://prosjektbanken.forskningsradet.no/en/project/FORISS/254822?Kilde=FORISS&distribution=Ar&chart=bar&calcType=funding&Sprak=no&sortBy=date&sortOrder=desc&resultCount=30&offset=180&ProgAkt.3=HAVBRUK2-Stort%20program%20for%20havbruksforskning Summary "Feed for farmed fish consists nowadays mostly of plant ingredients because there are not enough marine raw materials to cover the growing demand. Plants commonly used in fish feed are wheat and legumes like soy and pea. The significant changes in the feed can have consequences for the fish and for the consumers of fish-based food. The provision of the fish with enough nutrients has been achieved through the production of innovative feed compositions. In contrast, potential effects on consumers have been less investigated. Thus, the Norwegian Scientific Committee for Food Safety (VKM) has recently pointed out in an updated benefit-risk assessment of fish and fish products that there is a need to find out if some plant ingredients might be transferred from the feed to the edible parts of fish, which could be a risk for human health.
The SafeFish project will therefore examine if some typical plant ingredients can be carried over from feed to food. Three different substance groups will be analysed: potentially allergenic fragments of plant proteins (peptides), toxins produced by plant-colonising fungi (mycotoxins) and plant-produced compounds that resemble human hormones (phytoestrogens). Fish feed containing specific amounts of wheat, soy or pea will be produced and used in studies with zebrafish and on-growing farmed salmon under defined conditions in a research facility. Parts of the fish will be analysed with different methods to search for transferred substances, to look for possible changes in the biological functions of the fish, and to evaluate the potential health risk of consumers. The analytical methods used range from the specific detection of chemicals and proteins to DNA and RNA technologies and allergen testing in patients with food allergy.
No risk for consumers of fish produced with three plant-based feeds was found regarding allergenic peptides, mycotoxins and phytoestrogens, but a negative impact on fish health is possible, especially from enteropathological inflammation.
Specific outcomes:
- Development of 25-in-1 LC-HRMS/MS suitable for analysis of mycotoxins and phytoestrogens in feed and fish.
- Simultaneous characterisation of 5 major CYP P450 and 5 major UGT enzymes in liver fractions using specific substrates and LC-TripleQ-MS/MS analysis.
- Establishment of generally applicable salmon in vitro biotransformation assays using primary hepatocytes or liver microsomes.
- Novel sequential protease digestion method for wheat gluten sample preparation in proteomics.
- Development of label-free semi-quantitative nLC-nESI-MS/MS and quantitative LC-TripleQ-MS/MS for proteomics of gluten peptides in feed and fish.
- Workflows for the nutrigenomic and nutriepigenomic analysis of different fish tissues."
Keywords
Fish meal replacement;
Salmon;
Food quality;
Feed composition;
Fish;
Protein source;
Toxins;
Food safety;
Marine Region
76
Not associated to marine areas
0
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