Acronym RESILIENT SALMON
Category
Marine Biotechnology
Title Trained immunity and nutritional programming for resilient salmon Programme National Programme
Instrument (FP6)
Contact Type (FP7)
Strand (Interreg)
NA
Theme (FP7)
Activity Area (FP6)
Regional Area (Interreg)
Action (COST)
NA
Specific Programme (FP7)
NA
Funding source National Coordinator Margareth Øverland Coordinator email margareth.overland@nmbu.no
Coordinator institution
NMBU - Norwegian University of Life Sciences (Norway)
Institutions involved
NA
Start year 2019 End year 2024 Funding (€) € 1,009,030 Website https://prosjektbanken.forskningsradet.no/en/project/FORISS/294821?Kilde=FORISS&distribution=Ar&chart=bar&calcType=funding&Sprak=no&sortBy=date&sortOrder=desc&resultCount=30&offset=0&Organisasjon.3=STATISTISK%20SENTRALBYR%C3%85 Summary "Aquaculture is an important economic sector for Norway and the world. However, the continued growth of this industry has several challenges. RESILIENT SALMON will contribute to increased growth and value creation in the Norwegian salmon industry by developing a more robust fish with the ability to handle multi-stressor conditions (e.g., sub-optimal nutrition, diseases, and environmental problems) through an improved immuno-nutrition strategy. To achieve this, we combine in vitro screening experiments using salmon cells and in vivo feeding trials using fish exposed to microbe-associated molecular patterns (MAMPs), seawater transfer, pathogen outbreaks and short-term hypoxia.
Fish responses were documented using a multi-omic approach and by detection of specific biomarkers. The results showed that salmon cells induced with fucoidan from seaweed and microbial ingredients derived from yeasts (LAN4 and LAN6) were able to stimulate immune responses, activating and controlling the production of different biomarkers (e.g., cytokines and effector molecules), which may be crucial for fish homeostasis and welfare. Based on these data, we evaluated novel functional feeds for Atlantic salmon with the inclusion of these bioactive compounds.
In an acute hypoxia stress trial, LAN4 was able to modulate the fish´s physiological response, regulating the plasmatic secretion of cortisol and IL-10 (preventing immunosuppressive states), and increasing the levels of Muc-like proteins in the distal intestine, while in gills, the upregulation of metabolic pathways associated with stress-tolerance was detected. Furthermore, during an early natural outbreak of Moritella viscosa, which causes winter ulcers (skin wounds) in Atlantic salmon, vaccinated fish fed LAN6 showed a higher production of specific immunoglobulins against this pathogen, in addition to an upregulation of functional pathways associated with humoral immunity and complement activation in the liver. These results suggest that LAN6 could be a good candidate for functional feeds for salmon to achieve a more resilient fish against stressor conditions.
Functional feed containing fucoidan extracted from sugar kelp has shown promising effect on gut microbiota and immunomodulatory function in fish. For instance, fucoidan modulated the composition of gut microbiome in Atlantic salmon (increasing Bacillus spp), and led to an upregulation of genes related to lymph vessel morphogenesis, cell proliferation and differentiation, which may involve priming the immune system of Atlantic salmon by modulating T cell polarization in the distal intestine. This may be associated to the detection of with higher protein levels of cell-surface markers (e.g., CD3, CD8, and MCH-II), as well as the upregulation of gata3 (global transcriptional factor related to Th2-cell activation), which is crucial for antibody production and coordination of adaptive immune responses. In a follow-up trial, we have also detected that feed containing fucoidan improved growth performance and modulate trained immunity (by increasing the expression/production of antimicrobial peptides) in salmon against a challenge with MAMPs from an emerging bacterial pathogen (Tenacibaculum maritimum) during seawater stage. Taken together, use of functional feed with seaweed extracts and nutritional programming can help achieving a more robust salmon."
Keywords
Algae;
Bioactive compounds;
Bioprospecting;
Salmon;
Fish health;
Fish;
Animal feed;
Marine Region
76
Not associated to marine areas
0
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