Acronym NA
Category
Aquaculture
Title Tailored alginate scaffolds for low temperature 3D cultivation of primary fish cells Programme National Programme
Instrument (FP6)
Contact Type (FP7)
Strand (Interreg)
NA
Theme (FP7)
Activity Area (FP6)
Regional Area (Interreg)
Action (COST)
NA
Specific Programme (FP7)
NA
Funding source National Coordinator Øystein Arlov Coordinator email NA
Coordinator institution
SINTEF - SINTEF (Norway)
Institutions involved
NIFES - National Institute of Nutrition and Seafood Research (Norway) ,
NTNU - Norwegian University of Science and Technology (Norway) ,
Start year 2017 End year 2019 Funding (€) € 652,632 Website https://prosjektbanken.forskningsradet.no/en/project/FORISS/267585?Kilde=FORISS&distribution=Ar&chart=bar&calcType=funding&Sprak=no&sortBy=date&sortOrder=desc&resultCount=30&offset=150&TemaEmne.2=Marin+bioteknologi Summary The project aims to develop novel scaffolds of sulfated alginate hydrogels for 3D cultivation of primary fish cells at low temperatures; the Cabaret assay kit. The kit will sustain function and viability of cells for in vitro analyses in an in vivo-like environment. The scaffolds will be optimized for primary Atlantic salmon cells and integrated into standardized assay kits for each cell type. Proof-of-principle will be improved viability and function of cells in relevant environmental toxicity screening assays.
Salmon in aquaculture research constitute 87% of research animals used in Norway. Live fish experiments are costly and time-consuming, and 2D in vitro cell culture models existing today are too remote from in vivo conditions to replace animals, as the cells need to grow in a structured microenvironment to sustain functions. Commercially available scaffold materials cannot support viability and function of salmon cells, creating a market opportunity for novel materials. Sulfated alginate hydrogels have demonstrated great promise, and the Cabaret assay kits will allow the establishment of 3D in vitro assays as cost-effective alternatives to whole-animals in fish nutrition and toxicology research.
Toward this end, a library of functionalized alginates will be generated by SINTEF and NTNU, and evaluated as scaffold materials and cell culture substrates. NIFES will perform screening assays to identify the best scaffolds for each primary cell type, and the qualifying materials will be included in the Cabaret assay kits. If both 2D cell culture and live fish experiments are partially replaced by the Cabaret assay kit, it will be possible to sell 25,000 kits yearly, corresponding to annual sales of 50-100 million NOK.
Patents will be secured for developed materials, technology and/or applications, and for products and services. The project will proceed over two years, at an estimated budget of 6.2 million NOK for the partners SINTEF, NIFES, NTNU and SINTEF TTO
Keywords
Bioproduct;
Bioprospecting;
Fish;
Algae;
Salmon;
Marine Region
76
Not associated to marine areas
0
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