Acronym SALHEARTCELL
Category
Aquaculture
Title Development and use of primary and permanent salmonid heart cultures for detection and replication of piscine orthoreoviruses Programme National Programme
Instrument (FP6)
Contact Type (FP7)
Strand (Interreg)
NA
Theme (FP7)
Activity Area (FP6)
Regional Area (Interreg)
Action (COST)
NA
Specific Programme (FP7)
NA
Funding source National Coordinator Mikolaj Adamek Coordinator email mikolaj.adamek@tiho-hannover.de
Coordinator institution
TiHo - University of Veterinary Medicine Hannover (Germany)
Institutions involved
EMB - Fraunhofer Research Institution for Marine Biotechnology and Cell Technology (Germany) ,
Start year 2020 End year 2023 Funding (€) € 864,813 Website https://www.tiho-hannover.de/kliniken-institute/fachgebiete/abteilung-fischkrankheiten-und-fischhaltung/forschung-und-projekte/entwicklung-primaerer-zellkulturen-salheartcell Summary The aim of the SalHeartCell project is to develop primary cell cultures (Salmonid Cardiac Primary Cultures (SalCPCs)) and permanent cell cultures from salmonid cardiac cells. For this purpose, heart tissue from Atlantic salmon (Salmo salar ), rainbow trout (Oncorhynchus mykiss ) and brown trout (Salmo trutta) is used. The cell cultures developed are intended to be used to study viruses that can cause circulatory disorders in salmonids and multiply in heart tissue. The cell cultures will be thoroughly characterized in the project and used in experiments on replication and detection of piscine orthoreoviruses 1 and 3 (PRV-1 and PRV-3). PRV-1 and PRV-3 are newly emerging pathogens that endanger the sustainable production of salmonids in aquaculture, especially Atlantic salmon and rainbow trout, due to serious cardiovascular symptoms. The pathogens were recently detected in Germany for the first time. Despite various attempts, the cultivation of these viruses in existing cell lines has so far failed. This affects the diagnosis of the disease, the study of the viruses themselves and the development of vaccines significantly. SalCPCs contain spontaneously contracting cardiomyocytes, which could be one of the target cell types of PRV-1 and PRV-3. The further development of heart cell cultures could also be a very valuable tool for further studies on salmonids and heart tissues in general. The PRV-1 and PRV-3 cultivation trials are supported by external international partners from Norway, Canada and Denmark and aim to develop a proof-of-concept for SalCPCs as a test system for application in PRV-1 and PRV.
Keywords
Trout;
Salmon;
Fish health;
Disease;
Fish;
Marine Region
76
Not associated to marine areas
0
Marine Region Map
