Acronym NA
Category
Aquaculture
Marine Biotechnology
Fisheries
Title Effects of produced water to Atlantic cod: Mechanistic studies and biomarker development with proteomics based methods
Programme National Programme
Instrument (FP6)
Contact Type (FP7)
Strand (Interreg)
NA
Theme (FP7)
Activity Area (FP6)
Regional Area (Interreg)
Action (COST)
NA
Specific Programme (FP7)
NA
Funding source National
Coordinator Anders Goksoyr
Coordinator email Anders.Goksoyr@bio.uib.no
Coordinator institution
UiB - University of Bergen (Norway)
Institutions involved
IMR - Institute of Marine Research (Norway) ,
RCN - Research Council of Norway (Norway) ,
Start year 2005
End year 2008
Funding (€) € 381,252
Website NA
Summary The effects contaminants have on an organism have a molecular basis, and are transferred to higher levels of biological organization if the exposure is persistent and toxicological repair is overwhelmed. Long-term effects of produced water on the reproduction of Atlantic cod can, therefore, be studied at the molecular level. This project aims at utilising proteomics based techniques to study and identify possible changes in protein expression due to such exposure. We wanted to further develop proteomics based technology, i.e. 2-dimensional gel electrophoresis (2-DE) combined with different mass spectrometry techniques and database search, to identify mechanisms that occur due to exposure of Atlantic cod to produced water from oil and gas production. An important aim was to develop new biomarkers to be used in future biomonitoring programmes. 17 new cod specific antibodies to biomarker candidates have been developed. These are now being validated on material from laboratory experiments and field samples.
(1) To identify changes in protein expression due to exposure to produced water; (2) To develop proteomics-based technology to identify mechanisms of action; (3) To develop new biomarkers to be used in future biomonitoring programmes.
Keywords
Fish;
Monitoring;
Toxic substances;
Cod;
Impacts;
Marine Region
13
Northern North Sea (27.IVa)
1
Marine Region Map