Acronym NA
Category
Marine Biotechnology
Title Improved methodologies for harvest and purification of algal toxins Programme National Programme
Instrument (FP6)
Contact Type (FP7)
Strand (Interreg)
NA
Theme (FP7)
Activity Area (FP6)
Regional Area (Interreg)
Action (COST)
NA
Specific Programme (FP7)
NA
Funding source National Coordinator Morten Sandvik Coordinator email morten.sandvik@vetinst.no
Coordinator institution
NA
Institutions involved
NA - Not available (Sweden) ,
NA - Not available (New Zealand) ,
NA - Not available (Ireland) ,
Start year 2005 End year 2009 Funding (€) € 601,125 Website https://prosjektbanken.forskningsradet.no/en/project/FORISS/164851?Kilde=FORISS&distribution=Ar&chart=bar&calcType=funding&Sprak=no&sortBy=date&sortOrder=desc&resultCount=30&offset=330&TemaEmne.2=Fiskeri Summary Methods for large scale harvest of toxic algae and algal toxins by pumping seawater through plankton nets and columns with adsorbent materials will be developed. Furthermore one will develop methods for enzymatic conversion of algal toxins into toxic metabolites. Clean up methods for toxins from algal cultures, sea water, shellfish and crude extracts of metabolised toxins involving fewer steps, less use of solvents and the use of more environmental friendly solvents than used in exciting methods will be developed. The structure of the purified compounds will be elucidated using NMR in combination with data HPLC-MS analysis. The purified toxins will be used as standards in chemical analysis of shellfish. By increasing the availability of standards, new chemical methods can be developed and validated more rapidly, thus resulting in more rapid decrease in the use of the mouse bioassay test which is widely used in testing of shellfish today. More materials will be made available for toxicity testing of algal toxins and toxin analogues. The project includes training of one female PhD candidate. Furthermore it will result in increased collaboration between those Norwegian institutions already conducting research on algal toxins and it follows up the collaboration established in the project NFR 139593/140. Moreover, it creates added-value to the EU funded "BIOTOX" project (contract negotiated June 2004) by scaling up the production of materials that can be used as standards in chemical analysis of shellfish. The results from the project will place Norwegian researchers in the forefront in marine biotoxin research.
Primary objectives: (1) To build competence and capacity in algal toxin chemistry; (2) To develop efficient methodologies for large scale harvest and purification of algal toxins in order to provide laboratories with certified standards to be used in chemical analysis of shellfish for lipophilic marine biotoxins which will result in reduced need for the mouse bioassay test in the testing of shellfish. Secondary objectives: (1) Increase the Norwegian competence and capacity in the area marine biotoxins securing Norway a future role as a leading research nation in the field; (2) Develop methods emphasising on reduced steps in the methods, reduced use of materials, solvents and support for large scale harvest and purification for the following algal toxin groups - Dinophysis toxins (DTXs, including OA and OA-esters) - Yessotoxins (YTXs) - Azaspiracids (AZAs) - Pectenotoxins (PTXs); (3) Isolate, purify and elucidate the structure of "new" toxins or toxin analogues, and produce sufficient quantities of the novel compounds to test their toxicity; (4) Create added-value to the EU funded "BIOTOX" project by scaling up the production of materials that can be used as standards in chemical analysis of shellfish. Thus more extensive validation studies can be performed across Europe securing the validity of the new methodologies, which again will result in a more rapid decrease in the need for the mouse-bioassay test.
Keywords
Shellfish;
Algal toxins;
Toxic substances;
Biotechnology;
Marine Region
41
Norwegian Sea (27.IIa)
1
Marine Region Map
