Acronym NA
Category
Marine Biotechnology
Title Transcriptome sequencing of Atlantic salmon pituitary to identify novel genes involved in pubertal activation Programme National Programme
Instrument (FP6)
Contact Type (FP7)
Strand (Interreg)
NA
Theme (FP7)
Activity Area (FP6)
Regional Area (Interreg)
Action (COST)
NA
Specific Programme (FP7)
NA
Funding source National Coordinator Eirill Ager-Wick Coordinator email NA
Coordinator institution
NMBU - Norwegian University of Life Sciences (Norway)
Institutions involved
NA
Start year 2015 End year 2019 Funding (€) € 251,200 Website https://prosjektbanken.forskningsradet.no/project/FORISS/243811?Kilde=FORISS&distribution=Ar&chart=bar&calcType=funding&Sprak=no&sortBy=date&sortOrder=desc&resultCount=30&offset=240&TemaEmne.2=Marin+bioteknologi Summary To prevent the recurring problem of early puberty in the salmon industry, understanding the underlying molecular mechanisms is essential. The recently completed salmon genome has enabled a global approach to understand how cells or tissues regulate all prospective proteins (transcripts). In this project I will use a transcriptomics approach (RNA-seq) in combination with functional genomic tools to identify which genes are instrumental in deciding when an individual fish will enter pubertal development. The primary aim of this project is to identify (novel) genes that are important for pubertal activation of the pituitary, using male salmon as model. State-of-the-art genomic, bioinformatic and biotechnological methods will be implemented to elucidate pituitary mechanisms contributing to puberty. The transcriptome of male salmon pituitary will be characterized at the pre-, mid- and post-pubertal stage. Candidate genes identified through bioinformatics analyses will be investigated for functionality both in vivo and in vitro. The proposed project fits very well with my experience, having spent most of my PhD developing tools to investigate differentially expressed genes in eel and medaka pituitary cells using RNA-seq and functional studies of candidate genes. It is also complimentary to ongoing activities in my host groups at the Norwegian University of Life Sciences and Institute of Marine Research, thus complementing and strengthening the results obtained, and thereby enhancing the available tools and knowledge platform for improved salmon farming. The information acquired in this project should be very useful as direct targets for improved breeding and farming protocols in the salmon industry.
Keywords
Genomic sequencing;
Salmon;
Genetic;
Fish;
Marine Region
76
Not associated to marine areas
0
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