Acronym NA
Category
Seafood Processing
Title Standardisering av måling av pigment i muskulatur hos laksefisk - Determination of pigments in fish flesh by HPLC Programme National Programme
Instrument (FP6)
Contact Type (FP7)
Strand (Interreg)
NA
Theme (FP7)
Activity Area (FP6)
Regional Area (Interreg)
Action (COST)
NA
Specific Programme (FP7)
NA
Funding source National Coordinator Marianne Osterlie Coordinator email marianne.osterlie@hist.no
Coordinator institution
NTNU - Norwegian University of Science and Technology (Norway)
Institutions involved
NA
Start year 2007 End year 2009 Funding (€) € 55,917 Website https://www.fhf.no/prosjekter/prosjektbasen/573044/ Summary Pigments are important ingredients in feed to attain the wanted colour in salmon, and they are very costly raw materials. In 1994 a Norwegian standard (NS 9402) was established to measure colour and fat in Atlantic salmon. The initiative to this standard was worked out by a committee consisting of representatives from the export industry. NS 9402 does not determine which method should be used by chemical measurement of colour. Chemically measured pigmentation is required as a part of the documentation when salmon is sold. The presently dominating methods for colour measurement in Norwegian aquaculture industry are: (1) Visible light- (VIS)-spectroscopy; (2) Colour fan / Colour measurement instrument; (3) Fast methods (PhotoFish, NIR) with HPLC as reference method; (4) HPLC is to a great extent used in connection with R&D, but less as a routine method in quality control of salmon. The different methods of analysis mentioned above, can give quite large variation. Even within the same method of analysis variation will occur (similar to the variation found in pigment measurement of fish feed). Due to this, standardization of pigment measurement in salmon is required. Method of reference: There is analysis by HPLC which is suitable as a method of reference when quantifying pigment in salmon, as this is the only method which can differentiate between - and quantify - different carotenoids and isomers of these in fish muscle. The project is organized in a similar manner as the project for standardizing method for analysis of astaxathin in fish feed. The following components will be included: Invitation to stakeholders to participate in a standardization group. (In connection with the work the participants/businesses cover their own expenses). The establishment of a standardization group with 10-12 members Working out an account of the variation in the present methods for analysing pigment in salmon muscle Meet all interested parties in person, and thereafter meet with the standardization group to determine the requirements for a standardized method Working out a description and determination of method based on consensus Meet with stakeholders in person to discuss method and the organization of ring tests It does not seem very realistic to demand that the HPLC method should be standardized as a routine method by quality control of salmon if capacity, price and speed are taken into consideration. It is not desired to settle for one method as the only routine method, as development of new and improved fast methods is considered to be positive for the industry. One requirement, however, must be that all other methods should be calibrated and standardized against the reference method, and routinely be validated against the reference method through ring tests.
The Scope of the project was to specify a reference method for the determination of astaxanthin and canthaxanthin in salmonid fish flesh by high performance chromatography (HPLC). The method should be applied at a range above 0.02 mg pigment/kg. For sampling whole fish fillets the Norwegian quality cut (NS 9401.E) and the Norwegian quality standard preparation should be used (NS 9404
Keywords
Salmon;
Fish;
Marine Region
41
Norwegian Sea (27.IIa)
1
Marine Region Map
