Acronym In Vitro Fish
Category
Aquaculture
Title In Vitro Fish: New 3D models for nutritional and toxicological research Programme National Programme
Instrument (FP6)
Contact Type (FP7)
Strand (Interreg)
NA
Theme (FP7)
Activity Area (FP6)
Regional Area (Interreg)
Action (COST)
NA
Specific Programme (FP7)
NA
Funding source National Coordinator Liv Ingeborg Søfteland Coordinator email NA
Coordinator institution
IMR - Institute of Marine Research (Norway)
Institutions involved
NA
Start year 2015 End year 2018 Funding (€) € 442,480 Website https://prosjektbanken.forskningsradet.no/en/project/FORISS/244507?Kilde=FORISS&distribution=Ar&chart=bar&calcType=funding&Sprak=no&sortBy=date&sortOrder=desc&resultCount=30&offset=30&ProgAkt.3=HAVBRUKS-Havbruk+-+en+n%C3%A6ring+i+vekst Summary Future feeds for farmed fish are based on untraditional feed ingredients, which will change nutrient and contaminant profiles compared to traditional feeds based on marine ingredients. In fish nutrition and toxicology research, we aim to reduce the number of fish, refine the in vitro assays to better mimic the in vivo situation, and replace traditional fish feeding experiments. In vitro assays represent a more ethical way of conducting large-scale assessments of nutritional requirements and contaminant and mixture toxicity (cocktail effect). However, for in vitro models to reduce or replace the number of fish used in in vivo studies, it is critical to develop better and more advanced in vitro models resembling in vivo conditions to overcome the limitation of today's 2D models. To close the gap between the commonly used 2D mono cell culture assays and whole animal testing, the project In Vitro Fish proposes to refine existing in vitro methods and develop novel 3D Atlantic salmon cultures; co-culture, triple culture and a fish "organs-on-a-plate" culture, using primary hepatocytes, adipocytes and head kidney and kidney epithelial cells isolated from the same fish. These new in vitro models permit the study of animal physiology in a more organ-specific, in vivo like context that will replace and refine existing methodology and reduce the number of fish used in nutritional and toxicological research. The project has succeeded in improving existing cell methods and developing new 3D salmon cultures; co-cultures, triple cultures, and a circulation culture, using primary cells from the liver, adipose tissue, head kidney, and kidney isolated from the same fish. These models will be utilized in future projects at the Institute of Marine Research. The In Vitro Fish project further led to a collaboration with the Bergen Aquarium, which we have maintained throughout the project period. The Aquarium has housed the fish used in our experiments in an exhibition aquarium. Alongside the exhibition aquarium, information has been provided to the public that the fish are being used to develop new cell models and why this is important. The In Vitro Fish project uncovered a commercial shortage of good products for 3D cultivation of fish cells. Consequently, this resulted in a collaboration with SINTEF on a new NFR project where we will develop a new 3D cultivation product for fish cells.
Keywords
Fish;
Salmon;
Fish biology;
Diets;
Marine Region
76
Not associated to marine areas
0
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