Acronym NA
Category
Aquaculture
Title Development of methodologies for obtaining good quality spawning using artificial reproduction in brill (Scophthalmus rhombus): Physiological aspects Programme National Programme
Instrument (FP6)
Contact Type (FP7)
Strand (Interreg)
NA
Theme (FP7)
Activity Area (FP6)
Regional Area (Interreg)
Action (COST)
NA
Specific Programme (FP7)
NA
Funding source National Coordinator Gonzalo Martinez Rodriguez Coordinator email gonzalo.martinez@icman.csic.es
Coordinator institution
CSIC - Spanish National Research Council (Spain)
Institutions involved
IFAPA - Andalusian Institute of Agricultural Research and Training , Fisheries , Food and Organic Production (Spain) ,
INIA - National Institute of Agricultural and Food Research and Technology (Spain) ,
Start year 2007 End year 2010 Funding (€) € 63,074 Website NA Summary Water In Pino CIFAP working in collaboration with other institutions in the reproduction and breeding of Parracho (Scophthalmus rhombus) since 2002. In these four years has managed to reproduce the Parracho in captivity and successfully complete larval rearing stages and fattening (Torchholder et al., 2005). Despite these advances there are still aspects of the culture that require a more detailed study, which would give solutions to any issues that do not currently possible industrial scale cultivation. Among these issues include those related to reproduction. Preliminary studies carried out in this centre have found significant variability in fertility and the quality of the obtained set (Torchholder et al., In press). This variability does not ensure the availability of larval quality in sufficient quantity to Parracho growing at a breeding centre is economically viable. The variability of quality can be set due to different factors. In other fish species variations in quality parameters are related to setting the lipid composition of eggs (Devauchelle et al., 1982), which in turn is directly related to the diet of broodstock. In Parracho found correlations between some lipid classes (cholesterol and wax esters and cholesterol) and quality parameters set as the hatching rate (Torchholder et al., In press). Other causes which produce differences in the quality of the setting, primarily species that reproduce by artificial insemination, is the effect of the ripening of the eggs caused by the mismatch between the rates of ovulation and extraction frequency and variability sperm quality. These phenomena and their relationship to the quality of commissioning have been described in other species of flatfish such as turbot (Scophthalmus maximus) and halibut (Hippoglossus hippoglossus) (Fauvel et al., 1992, McEvoy et al., 1984; Norbreg et al., 1991, Cal et al. 1995; Suquet et al., 1995). Another important aspect of these species is the optimization of the method of fertilization and the determination of the optimum ratio egg: sperm (Cherenguini et al. 1991; Suquet et al., 1995). The work proposed in this project address the study of these four key points in the quality of a set, with the ultimate goal of designing a method of breeding flock management (feeding and extraction frequency gametes) and artificial insemination to ensure placed in sufficient quality for the crop Parracho feasible. In addition the project will evaluate the use of hormonal induction to the start as a complementary tool to improve the fertility and quality of the sunsets.
The general objective of the present project is to establish an adequate methodology for artificial reproduction of brill Scophthalmus rhombus. For this purpose, the reproductive biology of this species will be studied from two different points of view: (1) Zootechnical and nutritional issue; (2) Physiological issue The first task includes the study of the relationship between the methodology used for breeders handling (frequency of gamete extraction and feeding regime) and the parameters of spawning quality (fertilization and hatching rates, malformations rate, etc.). The relationship between the artificial fertilization technique and the fertilization rate will also be evaluated. The second task includes the study of the maturation-spawning cycles by mean of the analysis of plasma levels of several sex steroids and metabolites during such reproductive phenomena, as well as the evaluation and optimization of the use of available methods of hormonal treatments for spawning induction. Specific objectives: (1) Study of seasonal sexual maturation and ovulation and spermiation cycles In order to establish the periods of maturation and spawning and to study the ovulation and spermiation rhythms, the seasonal plasma levels of the main sex steroids and of other plasma metabolites will be quantified. The hormonal analyses will be accompanied by periodical biopsies to assess the oocyte development and stripping of the males to evaluate the quantity and quality of the sperm throughout the year. During the spawning period, the oocyte maturation stage will be related with the main parameters of spawning quality (fertilization and hatching rates, malformations rate, etc.); (2) Study of the methodology of artificial fertilization: determination of the optimal egg : sperm ratio. To analyse the relationship between the egg: sperm ratio used in the artificial fertilization and the fertilization rate obtained and thus to determinate the optimal gamete ratio which maximizes the fertilization rate; (3) Effect of broodstock feeding regime on spawning quality. To study the influence of the broodstock diet on the biochemical composition of the eggs as well as on egg quality (fertilization and hatching rates, malformations rate, etc.); (4) Relationship between the frequency of abdominal massage and spawning quality. To study the effects of the frequency of gamete extraction on plasma stress indicators (glucose, lactato, cortisol, etc.) and on spawning quality; (5) Evaluation of the use of different methods of hormonal treatment for spawning induction. To study the effectiveness to induce spawning and spermiation (both in a quantitative and a qualitative points of view) of a GnRH analogue developed for mammals used at different doses and applied using different systems of administration (injection and slow-release implants and microspheres).
Keywords
Flatfish;
Land-based aquaculture;
Fish;
Brill;
Fish reproduction;
Marine Region
76
Not associated to marine areas
0
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