Acronym NA
Category
Marine Biotechnology
Title Alphavirus (Togaviridae) fra laksefisk i norsk oppdrett. Karakterisering, vertikal overføring og studie av vevs/celle-tropisme - Alphavirus (Togaviridae) from salmonids in Norwegian farming. Characterization, vertical transfer and study of tissue / cell tropism Programme National Programme
Instrument (FP6)
Contact Type (FP7)
Strand (Interreg)
NA
Theme (FP7)
Activity Area (FP6)
Regional Area (Interreg)
Action (COST)
NA
Specific Programme (FP7)
NA
Funding source National
Coordinator institution
UiB - University of Bergen (Norway)
Institutions involved
NA
Start year 2005 End year 2010 Funding (€) € 164,114 Website https://prosjektbanken.forskningsradet.no/project/FORISS/164712?Kilde=EU&distribution=Ar&chart=bar&calcType=funding&Sprak=no&sortBy=date&sortOrder=desc&resultCount=30&offset=0&Prosjektleder=KONGSBERG+SEATEX+AS Summary Salmon pancreatic disease (SPD) was first described from Scotland in 1984, but was probably present in salmon farming in Scotland as early as 1976. In 1990, it was shown that the disease had an infectious cause. Later, in 1995, it became clear that the disease was caused by a togavirus-like agent that is about 65 nm in diameter. Based on RNA sequences from this virus, it has been suggested that it belongs to the alphatogavirus group in the Togaviridae family. In its typical form, the SPD starts with an acute outbreak 2 - 3 months after launch. The outbreak can be over in a couple of weeks or go into a chronic phase that can last for several months. Sick fish do not absorb food and after a while become lethargic, emaciated and dark-colored. The fish often swims sluggishly and high in the water before it dies. Mortality can vary from about 10% and up to 50%. The SPD is mainly registered for salmon in farming in western Norway, with some cases in northern Norway. The outbreak in northern Norway may be a result of the transport of smolts from western Norway to facilities in the north. Transport of SPDV via smolt supports a hypothesis that SPDV may have a freshwater reservoir or infection from broodstock and to smolts. This necessitates a closer investigation of the possible presence of SPDV in smolt production facilities. In this study, we will characterize the genome of alphavirus isolates, map the tissue and cell tropism of selected alphavirus isolates in acute disease and during a carrier state, in addition to investigating the possibility of vertical transmission of alphavirus. To investigate this we will use methods such as RNA purification, RT, Real-time RT-PCR, PCR and sequencing.
Keywords
Fish;
Genetic;
Genomic sequencing;
Parasite;
Disease;
Salmon;
Marine Region
76
Not associated to marine areas
0
Marine Region Map
